Methods for detecting dehydroepiandrosterone by mass spectrometry
AssigneeQuest Diagnostics Investments Incorporated
InventorsAmit Ghoshal, Nigel J. Clarke, Richard E. Reitz
Life of this patent
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Abstract
Provided are methods for determining the amount of underivatized dehydroepiandrosterone (DHEA) in a sample using mass spectrometry. The methods generally involve ionizing DHEA in a sample and detecting and quantifying the amount of the ion to determine the amount of DHEA in the sample.
Claims32
A method for determining the amount of underivatized dehydroepiandrosterone (DHEA) in a test sample, said method comprising:
- a. ionizing DHEA from said test sample to produce one or more DHEA ions detectable by mass spectrometry, wherein said ions comprise one or more ions selected from the group consisting of ions with a mass/charge ratio of 253.10±0.50, 197.13±0.50, and 167.10±0.50;
- and
- b. detecting the amount of one or more DHEA ions by mass spectrometry, wherein the amount of the DHEA ion(s) is related to the amount of underivatized DHEA in said test sample.
A method for determining the amount of underivatized dehydroepiandrosterone (DHEA) in a test sample by mass spectrometry, said method comprising:
- a. purifying DHEA in said test sample with high turbulence liquid chromatography (HTLC);
- b. ionizing DHEA from said test sample to produce one or more DHEA ions detectable by mass spectrometry;
- and
- c. detecting the amount of one or more DHEA ions by mass spectrometry, wherein the amount of the DHEA ion(s) is related to the amount of underivatized DHEA in said test sample.
A method for determining the amount of underivatized dehydroepiandrosterone (DHEA) in a test sample by tandem mass spectrometry said method comprising:
- a. purifying DHEA from said test sample by high turbulence liquid chromatography (HTLC);
- b. generating a precursor ion of said DHEA having a mass/charge ratio of 253.10±0.50;
- c. generating one or more fragment ions of said precursor ion, wherein at least one of said one or more fragment ions comprise a fragment ion selected from the group of fragment ions having a mass/charge ratio of 197.13±0.50 and 167.10±0.50;
- and
- d. detecting the amount of one or more of said ions generated in step (b) or (c) or both and relating the detected ions to the amount of underivatized DHEA in said test sample.
Dependencies are inferred from the claim text.