Cross-linked cellulose membranes
AssigneeGe Health Care Bio-Sciences AB
InventorsAsa Rudstedt, Andreas Axen, Nils Norrman, Anders Larsson
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Abstract
The invention relates to a process for making porous cross-linked charged cellulosic polymeric membranes capable of binding to a target molecule. The invention provides methods for separating target molecules from other components in a solution comprising use of membranes obtainable by the process of the invention. The method has particular utility in separating proteins and nucleic acids from cell lysates and cultures.
Claims24
A process for making a porous cross-linked charged cellulosic polymeric membrane comprising;
- i) adding a base to a porous cellulosic polymeric membrane in the presence of a solution of a bifunctional reagent to cross-link the cellulosic polymer therein,
- ii) reacting said membrane with a reagent that introduces a ligand, said ligand comprising a positively or negatively charged group
- wherein the resulting porous cross-linked charged cellulosic polymeric membrane swells from 40% to 250% by volume in a solution of water.
2-3. (canceled)
7-8. (canceled)
10-13. (canceled)
A porous cross-linked charged cellulosic polymeric membrane prepared by the process comprising;
- i) adding a base to a porous cellulosic polymeric membrane in the presence of a
- solution of a bifunctional reagent to cross-link the cellulosic polymer therein,
- ii) reacting said membrane with a reagent that introduces a ligand, said ligand comprising a positively or negatively charged group
- wherein the resulting porous cross-linked charged cellulosic
- polymeric membrane swells from 40% to 250% by volume in a solution of water.
26-27. (canceled)
Dependencies are inferred from the claim text.