Novel analytical method for protein

Abstract

Disclosed is a method of analyzing a protein, comprising the steps of: (A) labeling small amounts of protein samples with CyDyes having different fluorescent properties, respectively; (B) mixing 50-100 μg of each of the protein samples, labeled in step (A), with 1-5 mg of each of one or more of the unlabeled protein samples, and subjecting the mixture to electrophoresis; (C) subjecting the electrophoresed gel to fluorescence analysis to detect a difference in protein distribution between the protein samples; and (D) excising spot(s), showing the difference in protein distribution, from the gel, and isolating and identifying from the spots a protein, which is different in protein distribution between the protein samples. According to the present invention, a protein from a gel can be identified by MALDI-TOF, while simultaneously analyzing the gel qualitatively and quantitatively using a difference in the fluorescence of CyDyes with very high sensitivity.

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