Virosomes, methods of preparation, and immunogenic compositions
OwnerRichard W. Compans, Chinglai Yang +2inventors
InventorsRichard W. Compans, Chinglai Yang, Qizhi Yao, Sang-moo Kang
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Abstract
Briefly described, virosomes, methods of preparing virosomes, immunogenic compositions that include virosomes, and methods of eliciting an immune response using immunogenic compositions that include virosomes are described herein. A virosome can include at least one viral surface envelope glycoprotein expressed on the surface of the virosome. The virosome can also optionally include at least one adjuvant molecule expressed on the surface of the virosome.
Claims36
A chimeric virosome, comprising at least one surface molecule selected from the following:
- at least one chimeric viral surface envelope glycoprotein;
- at least two viral surface envelope glycoproteins expressed on the surface of the virosome, wherein at least one of the viral surface envelope glycoproteins is from a different virus than at least one other viral surface envelope glycoprotein;
- and
- at least one viral surface envelope glycoprotein and at least one adjuvant molecule, wherein at least one viral surface envelope glycoprotein and at least one adjuvant molecule are from different sources.
A chimeric virosome, comprising:
- at least one viral surface envelope glycoprotein expressed on the surface of the virosome;
- and
- at least one adjuvant molecule expressed on the surface of the virosome.
A method of producing a chimeric virosome in a host comprising:
- providing one or more expression vectors, wherein the one or more expression vectors comprise a polynucleotide sequence encoding for at least one viral surface envelope glycoprotein, and a polynucleotide sequence encoding for at least one adjuvant molecule;
- introducing the one or more expression vectors into a host cell;
- and
- expressing the at least one viral surface envelope glycoprotein and the at least one adjuvant molecule, whereby the virosome is formed by the cell in a host.
A method of immunizing a host comprising:
- co-expressing at least one viral surface envelope surface glycoprotein and at least one adjuvant molecule in one or more host cells;
- whereby the at least one viral surface envelope glycoprotein and at least one adjuvant molecule assemble to form a virosome.
An immunogenic composition, comprising a RVFV glycoprotein virosome and a pharmacologically acceptable carrier.
An immunogenic composition, comprising an Ebola glycoprotein virosome and a pharmacologically acceptable carrier.
An immunogenic composition, comprising a chimeric HIV Env protein virosome and a pharmacologically acceptable carrier.
Dependencies are inferred from the claim text.