Fermentation and purification of actinomadura chromoprotein and related species
AssigneeWyeth
InventorsEugene Joseph Vidunas, Gow-Jen Tsai, Ping Cai, Justin Moran, Pamela Sue Fink, Lourdes Jeanette Gordon
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Abstract
The present invention provides methods for production and purification of active chromoproteins produced by Actinomadura sp. 21G792. The chromoproteins are useful for developing pharmaceutical compositions and treating diseases such as cancer or bacterial infections.
Claims17
A fermentation culture comprising an actinomycete that produces a chromoprotein, a halide selected from bromide and iodide, and a surfactant selected from Pluronic L-61, Pluracol P2000 (polypropylene glycols) and Antarox 17-R2.
A method of producing a chromoprotein comprising fermenting Actinomadura sp. 21G792 in a medium comprising a halide selected from bromide and iodide, and a surfactant selected from Pluronic L-61, Pluracol P2000 (polypropylene glycols) and Antarox 17-R2.
A method of purifying a chromoprotein of Actinomadura sp. 21G792 comprising obtaining a fluid comprising the Actinomadura sp. 21G792 chromoprotein, and contacting the fluid with one or more of
- a) an anion exchange chromatography matrix,
- b) a hydrophobic interaction chromatography matrix, or
- c) a size exclusion chromatography matrix,
- and recovering the purified chromoprotein.
A method of purifying a chromoprotein of Actinomadura sp. 21G792 comprising:
- (a) obtaining a fluid comprising the Actinomadura sp. 21G792 chromoprotein, (b) contacting the fluid with an anion exchange chromatography matrix, (c) contacting the fluid with a hydrophobic interaction chromatography matrix, (d) contacting the fluid with a size exclusion chromatography matrix, and (e) recovering the purified chromoprotein.
-20. (canceled)
Dependencies are inferred from the claim text.