Bacterial replication systems and methods
AssigneeReplidyne, Inc.
InventorsThale Cross Jarvis, Charles S. McHenry, Nebojsa Janjic, Garry Dallmann, James M. Bullard, Stacie Bell, Glenn Sanders, Frank Dean
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Abstract
Reconstituted bacterial replication assemblies and methods for their use are provided.
Claims47
A method for identifying a compound that modulates the activity of a bacterial replication assembly, comprising:
- a) contacting a DNA molecule capable of being replicated with a bacterial replication assembly with a test compound to form a reaction mixture;
- b) subjecting the reaction mixture to conditions, which in the absence of the compound, effect nucleic acid polymerization;
- and
- c) comparing the nucleic acid polymerization in the presence of the test compound with the nucleic acid polymerization in the absence of the test compound, wherein a change in the activity of the bacterial replication assembly in the presence of the test compound is indicative of a compound that modulates the activity of the bacterial replication assembly.
A method for identifying a compound that modulates the activity of a bacterial replication assembly, comprising:
- a) contacting a each of a plurality of DNA molecules capable of being replicated with a bacterial replication assembly with a different test compound to form a plurality of reaction mixtures;
- b) subjecting the reaction mixtures to conditions, which in the absence of the compounds, effect nucleic acid polymerization;
- and
- c) comparing the nucleic acid polymerization in the presence of the test compounds with the nucleic acid polymerization in the absence of the test compounds, wherein a change in the activity of the bacterial replication assembly in the presence of any one compound is indicative of a compound that modulates the activity of the bacterial replication assembly.
A reconstituted bacterial replication assembly capable of functioning in a test for identifying compounds that modulate the ability of bacterial replication assembly to replicate DNA.
A method of synthesizing a DNA molecule comprising contacting a DNA molecule with a bacterial replication assembly and one or more dNTPs under conditions sufficient to synthesize a second DNA molecule complementary to all or a portion of said first DNA molecule, wherein said bacterial replication assembly is selected from the group consisting of an S. pyogenes bacterial replication assembly, an S. aureus bacterial replication assembly, a Y. pestis bacterial replication assembly, a P. aeruginosa bacterial replication assembly, a B. subtilis bacterial replication assembly, and an E. coli bacterial replication assembly.
Dependencies are inferred from the claim text.