Novel 2,5-diketo-l-gluconic acid reductases and method of use
AssigneeDanisco US Inc.
InventorsMARK DONNELLY, WILLIAM H. ESCHENFELDT, JONATHAN TRENT
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Abstract
Described herein are novel nucleic acids, proteins and methods that can be used to provide new catalysts with desirable traits for industrial processes. In particular, novel reductases isolated from the environment using PCR methods are described.
Claims24
An isolated nucleic acid molecule comprising a nucleic acid sequence which encodes a peptide having an amino acid sequence which has at least about 60% sequence identity to an amino acid sequence as set forth in FIG. 2A or 2B.
An isolated nucleic acid molecule which comprises a sequence having at least about 60% sequence identity to a sequence selected from the group of sequences set forth in FIG. 1.
An isolated polypeptide comprising an amino acid sequence having at least about 60% identity to an amino acid sequence as set forth in FIG. 2A or 2B, wherein said polypeptide comprises 2,5-diketo-D-gluconic acid reductase activity.
A method for identifying a 2,5-diketo-L-glucoric acid reductase comprising:
- a) isolating nucleic acid molecules having homology to 2,5-diketo-L-gluconic acid reductases from uncultured microorganisms;
- and
- b) screening said molecules for 2,5-diketo-D-gluconic acid reductase activity, wherein said molecules having 2,5-diketo-D-gluconic acid reductase activity are identified as a 2,5-diketo-L-gluconic acid reductase.
Dependencies are inferred from the claim text.