Systems for reducing biomass recalcitrance
AssigneeEdenspace Systems Corporation
InventorsKirk PAPPAN, Ramesh NAIR, Forrest CHUMLEY
Life of this patent
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Abstract
Improved systems and methods for reducing costs and increasing yields of cellulosic ethanol including compositions of matter comprising plant biomass and cell wall-modifying enzyme polypeptides and transgenic plants expression cell wall-modifying enzyme polypeptides.
Claims35
A composition of matter comprising plant biomass and at least one enzyme polypeptide having at least 85% amino acid sequence identity to at least one of SEQ ID NO:
- 1 to 84.
A transgenic plant, the genome of which is augmented with:
- a recombinant polynucleotide encoding at least one enzyme polypeptide linked to a promoter sequence,
- wherein the polynucleotide is optimized for expression in the plant, wherein the at least one enzyme polypeptide has at least 85% sequence identity to at least one of SEQ ID NO.:
- 1 to 84.
23-46. (canceled)
An expression vector comprising a nucleic acid encoding an enzyme polypeptide having at least 85% amino acid sequence identity to at least one of SEQ ID NO:
- 1 to 84.
48-50. (canceled)
A transformed cell comprising a nucleic acid encoding an enzyme polypeptide having at least 85% amino acid sequence identity to at least one of SEQ ID NO:
- 1 to 84.
52-53. (canceled)
A method comprising steps of:
- pretreating a plant part under conditions to promote accessibility of celluloses within the lignocellulosic biomass;
- and
- treating the pretreated plant part under conditions that promote hydrolysis of cellulose to fermentable sugars,
- wherein the plant part is obtained from at least one transgenic plant, the genome of which is augmented with:
- a recombinant polynucleotide encoding at least one enzyme polypeptide operably linked to a promoter sequence, wherein the polynucleotide is optimized for expression in the plant and wherein the at least one enzyme polypeptide has at least 85% sequence identity to at least one of SEQ ID NO.:
- 1 to 84.
An isolated antibody that binds specifically to a feruloyl esterase polypeptide.
(canceled)
An isolated antibody that binds specifically to an exoglucanase polypeptide.
58-60. (canceled)
An array comprising a solid substrate, the substrate having a surface, and a plurality of genetic probes
- wherein each genetic probe is immobilized to a discrete spot on the surface of the substrate to form an array, and
- wherein the plurality of genetic probes comprises at least ten different oligonucleotides, each oligonucleotide comprising at least ten consecutive nucleotides from a nucleic acid encoding a polypeptide have a sequence of one of SEQ ID NO:
- 1 to 84.
62-64. (canceled)
A plate comprising a solid substrate, the substrate having a surface, and a peptide immobilized to the surface, wherein the peptide comprises at least six consecutive amino acids from a polypeptide having a sequence of one of SEQ ID NO:
- 1 to 84.
Dependencies are inferred from the claim text.