Back to results
US 2010/0016697 A1Published application
Systems and methods for in vivo measurement of interstitial biological activity, processes and/or compositions
AssigneeMUSC Foundation for Research Developement
InventorsFrancis G. Spinale, Robert Stroud, Michael Looper, Anne Deschamps
Life of this patent
Estimating the term…
Abstract
Provided are systems and methods for detecting and/or measuring in vivo interstitial biological activity, processes, and or compounds in human or animal subjects.
Claims36
A detection system, comprising:
- at least one substrate having a first state that can be altered by enzymatic activity within a subject's interstitial fluid to a second detectable state;
- a detector apparatus comprising a substrate receptacle, an energy source and a detector element, wherein the substrate receptacle is in fluid communication with the at least one substrate in the second detectable state;
- and
- means for positioning the substrate receptacle, energy source and detector element within the detector apparatus such that energy generated by the energy source can be directed into the substrate receptacle to excite the detectable substrate and such that the excited detectable substrate can be detected by the detector element.
1 more element
5-6. (canceled)
(canceled)
(canceled)
(canceled)
A detection system, comprising:
- at least one fluorogenic substrate configured to interact with a compound of interest located within the interstitial fluid of a subject;
- a detector apparatus comprising a substrate receptacle, an energy source and a detector element, wherein the substrate receptacle is in fluid communication with the fluorogenic substrate and is configured to accept interstitial fluid comprising the substrate;
- and
- means for positioning the substrate receptacle, energy source and detector element within the detector apparatus such that energy generated by the energy source can be directed into the substrate receptacle to excite the accepted substrate and such that the excited substrate can be detected by the detector element.
1 more element
(canceled)
(canceled)
(canceled)
27-29. (canceled)
31-34. (canceled)
38-39. (canceled)
41-47. (canceled)
A method of real-time in vivo monitoring of a biological process, comprising:
- identifying a compound of interest that is located in the interstitium of the subject, wherein the activity or presence of the compound is related to the in vivo biological process being monitored;
- positioning a probe within the interstitium of the subject;
- infusing the interstitium of the subject with a fluorogenic substrate specific for the compound of interest wherein the substrate is infused therethrough the probe;
- receiving interstitial fluid from the subject with the probe, wherein the interstitial fluid comprises the substrate;
- and
- detecting the received substrate to monitor the biological process in the subject.
3 more elements
49-89. (canceled)
Dependencies are inferred from the claim text.