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AssigneeWisconsin Alumni Research Foundation
InventorFrancesco Cerrina
Estimating the term…
Synthesis of chain molecules such as DNA is carried out in a conduit having an interior channel with an inlet end and an outlet end. At least one wall of the conduit is substantially transparent to selected wavelengths of light. Solid carrier particles are contained within the interior channel of the conduit. A plurality of controllable light sources are mounted at spaced locations along the length of the transparent wall of the conduit to allow selective illumination of separated sections of the particles within the conduit. When a light source is turned on, a photodeprotecting group is removed from the carrier particles in the section that is illuminated by the light source. A reagent containing a selected base is flowed through the conduit so that the base will attach to the carrier particles in those sections which have been exposed to light and deprotected.
A method of synthesizing DNA chain molecules on carrier particles within an interior channel of a conduit, the method comprising:
The method of claim I wherein the conduit has walls which are all transparent to light and further including a material adjacent to the conduit between the optically isolated sections which blocks the transmission of light through the walls of the conduit to minimize stray light.
Dependencies are inferred from the claim text.