Whole blood-derived coagulum device for treating bone defects

A method of treating a bone defect in an individual in need of treatment thereof comprising:

• 1. preparing an osteogenic whole blood-derived coagulum device (WBCD) by the steps comprising:
• (a) combining:
• (i) whole blood,
• (ii) an osteogenic protein,
• (iii) exogenously provided calcium ion,
• and
• (iv) optionally, a fibrin-thrombin mixture,
• (b) incubating the ingredients combined in step (a) until a mechanically stable coagulum is formed,
• wherein the exogenously provided calcium ion is present at a concentration that is effective to provide a homogeneous, cohesive, syringeable, injectable, and malleable coagulum gel,
• and
• 2. administering the WBCD of step 1 to the bone defect in the individual.

A kit for preparing a whole blood-derived coagulum device (WBCD) for treating a bone defect comprising:

• 1. a vial containing a lyophilized osteogenic BMP,
• 2. a buffer for reconstituting the lyophilized BMP powder,
• 3. a syringe and a needle for reconstituting the lyophilized BMP in the buffer,
• 4. a vacutainer for collecting a patient's blood,
• 5. a sterile solution of 1 M CaCl2,
• 6. a fibrin-thrombin mixture,
• 7. a plastic, siliconized container for mixing whole blood with the reconstituted BMP,
• 8. a spatula or a syringe for applying WBCD to bone ends during open surgery, and
• 9. instructions for the preparation and use of osteogenic WBCD using autologous or crossmatched blood.