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US 2009/0293151 A1Published application
Dgat genes from oleaginous organisms for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
AssigneeE. I. Du Pont de Nemours and Company
InventorsKNUT MEYER, HOWARD GLENN DAMUDE, KEVIN G. RIPP, KEVIN L. STECCA
Life of this patent
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Abstract
Transgenic soybean seed having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. DGAT genes from oleaginous organisms are used to achieve the increase in seed storage lipids.
Claims18
An isolated polynucleotide comprising:
- (a) a nucleotide sequence encoding a polypeptide having diacylglycerol acyltransferase activity wherein the polypeptide has at least 80% amino acid identity, based on the Clustal V method of alignment, when compared to an amino acid sequence as set forth in SEQ ID NOs:135, 136, 147, 162, 176, 215, 234, 265, 272, 279, 299, 304, 306, 308, 310, 312, 314, 316, 318, 320, 322, 351, or 363;
- (b) a nucleotide sequence encoding a polypeptide having diacylglycerol acyltransferase activity, wherein the nucleotide sequence has at least 80% sequence identity, based on the BLASTN method of alignment, when compared to a nucleotide sequence as set forth in SEQ ID NO:
- 133, 134, 146, 161, 175, 214, 233, 264, 271, 278, 298, 301, 303, 305, 307, 309, 311, 313, 315, 317, 319, 321, 350, or 352;
- (c) a nucleotide sequence encoding a polypeptide having diacylglycerol acyltransferase activity, wherein the nucleotide sequence hybridizes under stringent conditions to a nucleotide sequence as set forth in SEQ ID NO:
- 133, 134, 146, 161, 175, 214, 233, 264, 271, 278, 298, 301, 303, 305, 307, 309, 311, 313, 315, 317, 319, 321, 350, or 352;
- or
- (d) a complement of the nucleotide sequence of (a), (b) or (c), wherein the complement and the nucleotide sequence consist of the same number of nucleotides and are 100% complementary.
4 more elements
Dependencies are inferred from the claim text.