Method for the production of calendic acid, a fatty acid containing delta 8,10,12 conjugated double bonds and related fatty acids having a modification at the delta 9 position

A method of altering the level of fatty acids in a host cell or plant wherein said fatty acids comprise a modification at a delta-9 position, said method comprising:

• (a) transforming a host cell or plant with a chimeric gene comprising an isolated nucleic acid fragment encoding a plant fatty acid modifying enzyme associated with conjugated double bond formation comprising a delta-9 position of fatty acids having an amino acid identity of at least 72.5% based on the Clustal method of alignment when compared to a polypeptide of SEQ ID NO:13 wherein said fragment or a functionally equivalent subfragment thereof or a complement thereof is operably linked to at least one suitable regulatory sequence;
• (b) growing the transformed host cell or plant under conditions suitable for the expression of the chimeric gene;
• and
• (c) selecting those transformed host cells or plants having altered levels of fatty acids comprising a modified delta-9 position.

A method of altering the level of fatty acids in a host cell or plant wherein said fatty acids comprise a modification at a delta-9 position, said method comprising:

• (a) transforming a host cell or plant with a chimeric gene comprising an isolated nucleic acid fragment encoding a plant fatty acid modifying enzyme associated with conjugated double bond formation comprising a delta-9 position of fatty acids having an amino acid identity of at least 72.5% based on the Clustal method of alignment when compared to a polypeptide of SEQ ID NO:13 wherein said fragment or a functionally equivalent subfragment thereof or a complement thereof is operably linked to at least one suitable regulatory sequence and wherein said nucleic acid sequence is isolated from Dimorphotheca sinuate;
• (b) growing the transformed host cell or plant under conditions suitable for the expression of the chimeric gene;
• and
• (c) selecting those transformed host cells or plants having altered levels of fatty acids comprising a modified delta-9 position.

A method of altering the level of fatty acids in a host cell or plant wherein said fatty acids comprise a modification at a delta-9 position, said method comprising:

• (a) transforming a host cell or plant with a chimeric gene comprising an isolated nucleic acid fragment encoding a plant fatty acid modifying enzyme associated with conjugated double bond formation comprising a delta-9 position of fatty acids having an amino acid identity of at least 72.5% based on the Clustal method of alignment when compared to a polypeptide of SEQ ID NO:13 wherein said fragment or a functionally equivalent subfragment thereof or a complement thereof is operably linked to at least one suitable regulatory sequence and wherein said plant fatty acid modifying enzyme is associated with the formation of dimorphecolic acid;
• (b) growing the transformed host cell or plant under conditions suitable for the expression of the chimeric gene;
• and
• (c) selecting those transformed host cells or plants having altered levels of fatty acids comprising a modified delta-9 position.

A method for producing fatty acid modifying enzymes associated with modification of a delta-9 position of fatty acids which comprises:

• (a) transforming a microbial host cell with a chimeric gene comprising an isolated nucleic acid fragment encoding a plant fatty acid modifying enzyme associated with conjugated double bond formation comprising a delta-9 position of fatty acids having an amino acid identity of at least 72.5% based on the Clustal method of alignment when compared to a polypeptide of SEQ ID NO:13 wherein said fragment or a functionally equivalent subfragment thereof or a complement thereof is operably linked to at least one suitable regulatory sequence;
• (b) growing the transformed host cell under conditions suitable for the expression of the chimeric gene;
• and
• (c) selecting those transformed host cells containing altered levels of protein encoded by the chimeric gene.

A method for producing fatty acid modifying enzymes associated with modification of a delta-9 position of fatty acids which comprises:

• (a) transforming a microbial host cell with a chimeric gene comprising an isolated nucleic acid fragment encoding a plant fatty acid modifying enzyme associated with conjugated double bond formation comprising a delta-9 position of fatty acids having an amino acid identity of at least 72.5% based on the Clustal method of alignment when compared to a polypeptide of SEQ ID NO:13 wherein said fragment or a functionally equivalent subfragment thereof or a complement thereof is operably linked to at least one suitable regulatory sequence and wherein said nucleic acid sequence is isolated from Dimorphotheca sinuate;
• (b) growing the transformed host cell under conditions suitable for the expression of the chimeric gene;
• and
• (c) selecting those transformed host cells containing altered levels of protein encoded by the chimeric gene.

A method for producing fatty acid modifying enzymes associated with modification of a delta-9 position of fatty acids which comprises:

• (a) transforming a microbial host cell with a chimeric gene comprising an isolated nucleic acid fragment encoding a plant fatty acid modifying enzyme associated with conjugated double bond formation comprising a delta-9 position of fatty acids having an amino acid identity of at least 72.5% based on the Clustal method of alignment when compared to a polypeptide of SEQ ID NO:13 wherein said fragment or a functionally equivalent subfragment thereof or a complement thereof is operably linked to at least one suitable regulatory sequence and wherein said plant fatty acid modifying enzyme is associated with the formation of dimorphecolic acid;
• (b) growing the transformed host cell under conditions suitable for the expression of the chimeric gene;
• and
• (c) selecting those transformed host cells containing altered levels of protein encoded by the chimeric gene.