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AssigneesVereniging voor christelijk hoger onderwijs wetens chappelijk onderzoek en patientenzorg, International Flower Developoments Pty. Ltd.
InventorsFrancesca Quattrocchio, Ronald Koes, Walter Verweij, Kees Spelt, Filippa Brugliera, Masako Mizutani
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The present invention relates generally to the field of plant molecular biology and agents useful in the manipulation of plant physiological or biochemical properties. More particularly, the present invention provides genetic and proteinaceous agents capable of modulating or altering the level of acidity or alkalinity in a cell, group of cells, organelle, part or reproductive portion of a plant. Genetically altered plants, plant parts, progeny, subsequent generations and reproductive material including flowers or flowering parts having cells exhibiting an altered cellular pH compared to a non-genetically altered plant are also provided.
An isolated nucleic acid molecule comprising a sequence of nucleotides encoding or complementary to a sequence encoding a polypeptide having pH altering activity obtainable from rose wherein expression of said nucleic acid molecule alters the pH inside the cell.
A method for producing a transgenic flowering plant capable of synthesizing a pH modulating or altering protein, said method comprising stably transforming a cell of a suitable plant with a nucleic acid sequence obtainable from rose which comprises a sequence of nucleotides encoding said pH modulating or altering proteins under conditions permitting the eventual expression of said nucleic acid sequence, regenerating a transgenic plant from the cell and growing said transgenic plant for a time and under conditions sufficient to permit the expression of the nucleic acid sequence.
A method for producing a transgenic plant with reduced indigenous or existing pH modulating or altering activity, said method comprising stably transforming a cell of a suitable plant with a nucleic acid molecule obtainable from rose which comprises a sequence of nucleotides encoding or complementary to a sequence encoding a polypeptide having pH modulating activity, regenerating a transgenic plant from the cell and where necessary growing said transgenic plant under conditions sufficient to permit the expression of the nucleic acid.
A method for producing a genetically modified plant with reduced indigenous pH altering protein activity, said method comprising altering the pH by reducing expression of a gene encoding an indigenous pH altering protein by introduction of a nucleic acid molecule into a plant cell, and regenerating the genetically modified plant from the cell.
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A method for producing a transgenic plant capable of generating a pH altering protein, said method comprising stably transforming a cell of a suitable plant with the isolated nucleic acid molecule obtainable from rose comprising a sequence of nucleotides encoding, or complementary to, a sequence encoding the pH altering protein and regenerating a transgenic plant from the cell.
An isolated cell, plant or part of a genetically modified plant or progeny thereof which cell, plant or part comprises an altered pH in a vacuole of the cell or cells of the plant or plant parts.
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Dependencies are inferred from the claim text.