Zea mays ribulose bisphosphate carboxylase activase promoter
AssigneeMonsanto Technology LLC
InventorsKunsheng Wu, Santanu Dasgupta, Targolli Jayaprakash, Shoba Cherian
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Abstract
The present invention provides gene regulatory element polynucleotide molecules, including a promoter and a leader, identified from the ribulose bisphosphate carboxylase activase (RUA) Zea mays gene, useful for expressing transgenes in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds comprising the Zea mays gene regulatory molecules, and methods for preparing and using the same.
Claims31
A gene regulatory element molecule isolated or identified from Zea mays, or a complement thereof, or a fragment thereof, or a cis element thereof, wherein said gene regulatory element functions in transcriptional control for a ribulose bisphosphate carboxylase activase (RUA) gene.
A method of transforming a host cell comprising:
- a. providing a nucleic acid molecule that comprises in the 5′ to 3′ direction:
- a nucleic acid sequence that exhibits an 80% or greater identity to a sequences elected from the group consisting of:
- SEQ ID NO:
- 17666, or nucleotides 1553 through 2280 of SEQ ID NO:
- 17666, or nucleotides 2281 through 2325 of SEQ ID NO:
- 17666;
- or any complement thereof, or any fragment thereof, or any cis element thereof,
- b. or any complements thereof, operably linked to a transcribable polynucleotide molecule sequence, and
- c. transforming said cell with the nucleic acid molecule.
Dependencies are inferred from the claim text.