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US 2009/0293139 A1Published application
Double-inducible gene activation system and its applications
AssigneeTHE TEXAS A&M UNIVERSITY SYSTEM an Agency of the state of Texas
InventorsJiang Chang, Robert J. Schwartz, Viraj R. Shah
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Abstract
A double-inducible system for expressing a transgene, preferably comprising an RU486-inducible system integrated with a CID-inducible system. The invention further comprises a gene expression system for use in in vitro cell culture studies, and a gene expression expression system for use in engineering modified bigenic mice.
Claims23
A double-inducible gene expression system comprising:
- a) a first plasmid comprising a chimeric transcription factor;
- and
- b) a second plasmid comprising a transgene, the transgene comprising a target gene and an inducible dimerization domain, and wherein the chimeric transcription factor is capable of regulating expression of the transgene.
A double inducible gene expression system comprising:
- an inducible system at a transcriptional level;
- and
- an inducible system at a posttranslational level.
A method for generating a bigenic mouse comprising the steps of:
- a) obtaining a first transgenic mouse which expresses a first plasmid, the first plasmid comprising a chimeric transcription factor;
- and
- b) breeding the first transgenic mouse with a second transgenic mouse which expresses a second plasmid, the second plasmid comprising a transgene, the transgene comprising a target gene and an inducible dimerization domain;
- wherein the transcription factor is capable of reglating expression of the transgene.
1 more element
Dependencies are inferred from the claim text.