Rapid Detection of Anti-Chromatin Autoantibodies in Human Serum using a Portable Electrochemical Biosensor
OwnerRobert Rubin, Ravil Sitdikov +3inventors
InventorsRobert Rubin, Ravil Sitdikov, Plamen Atanassov, Gabriel Lopez, Konstantin Konstantinov
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Abstract
A rapid electrochemical flow-through sensor and method for detecting the presence of autoantibodies in a fluid sample such as blood is shown and described. The sensor may take either a single- or multi-plexed form. The sensor comprises a fluid inlet, a reaction region comprising immobilized autoantigens and an electrode assembly, and a fluid outlet.
Claims20
A method for detecting autoantibodies associated with an autoimmune disease in a human test sample comprising:
- providing an electrochemical flow-through sensor comprising:
- an inlet channel configured to deliver fluid into a reaction region;
- a reaction region comprising:
- an electrode assembly comprising a working electrode;
- and
- immobilized autoantigens configured to present the product of an enzymatic reaction to the electrode assembly so as to produce a detectable alteration in the working electrode's signal output;
- and
- an outlet channel configured to allow for fluid flow out of the reaction region, wherein the outlet channel is not the inlet channel;
- introducing the test sample to the reaction region via the inlet channel under sufficient conditions for autoantibodies in the test sample to bind to the immobilized autoantigens;
- allowing unbound molecules to exit the reaction region via the outlet channel;
- introducing one or more detection molecules configured to, upon interaction with the bound autoantibodies, produce an enzymatic reaction, the product of which is able to produce a detectable alteration in the working electrode's signal output.
A flow-through electrochemical apparatus for detecting autoantibodies associated with an autoimmune disease in a human test sample, the apparatus comprising:
- a housing containing:
- an inlet channel configured to deliver fluid into a reaction region;
- a reaction region comprising:
- an electrode assembly comprising a working electrode and a reference;
- and
- immobilized autoantigens configured to present the product of an enzymatic reaction to the electrode assembly so as to produce a detectable alteration in the working electrode's signal output;
- and
- an outlet channel configured to allow for fluid flow out of the reaction region, wherein the outlet channel is not the inlet channel.
A flow-through electrochemical apparatus for detecting autoantibodies associated with an autoimmune disease in a human test sample, the apparatus comprising:
- a housing containing:
- a plurality of inlet channel configured to deliver fluid into a plurality of reaction regions, wherein each reaction region comprises:
- an electrode assembly comprising a working electrode and a reference;
- and
- immobilized autoantigens configured to present the product of an enzymatic reaction to the electrode assembly so as to produce a detectable alteration in the working electrode's signal output;
- and
- a plurality of outlet channels configured to allow for fluid flow out of the reaction regions, wherein the outlet channels are not the inlet channels.
Dependencies are inferred from the claim text.