Method for Automated High Throughput Identification of Carbohydrates and Carbohydrate Mixture Composition Patterns as well as Systems Therefore

A method for an automated carbohydrate mixture composition pattern profiling comprising the steps of

• a) providing a sample containing a carbohydrate mixture composition;
• b) labelling of said carbohydrate mixture composition with a fluorescent label;
• c) providing a second sample having a known carbohydrate mixture composition pattern to be compared with
• d) generating electropherograms of the carbohydrate mixture composition of the first and second sample using capillary gel electrophoresis-laser induced fluorescence;
• e) comparing the obtained electropherogram or the migration times calculated therefrom of the first sample and the second sample;
• f) analysing the identity and/or differences between the carbohydrate mixture composition pattern profiles of the first and the second sample.

Database containing normalized migration times and or normalized migration time indices of carbohydrates, said migration times and/or migration time indices are obtained by

• a) analysing known carbohydrate structure(s) in a sample together with an internal standard at the same time with a capillary gel electrophoresis-laser induced fluorescents means;
• b) determining the migration time(s) of said carbohydrate compound(s) and the standard;
• c) normalizing the migration time(s) of step b.) based on given migration time(s) of the internal standard;
• d) assigning the normalized carbohydrate compound(s) migration time(s) to standard migration time indices.