Methylbutanol as an advanced biofuel
AssigneeSynthetic Genomics, Inc.
InventorsStephen Picataggio, Robert Christopher Brown, Jessica Roxane Kristof, Gena Lee, Prachee Prakash, Stuart Alan Underwood, Kevin Watts
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Abstract
This invention describes genes, metabolic pathways, microbial strains and methods to produce methyl butanol and other compounds of interest from renewable feedstocks.
Claims96
A recombinant microorganism comprising at least one DNA molecule, wherein said at least one DNA molecule encodes at least three polypeptides that catalyze a substrate to product conversion selected from the group consisting of:
- a) malate to pyruvate,
- b) pyruvate to oxaloacetic acid,
- c) oxaloacetic acid to L-aspartate,
- d) L-aspartate to L-aspartyl-4-phospate,
- e) L-aspartyl-4-phospate to L-aspartate-semialdehyde,
- f) L-aspartate-semialdehyde to homoserine,
- g) homoserine to O-phospho-L-homoserine, and
- h) O-phospho-L-homoserine to L-threonine;
- and wherein said recombinant microorganism produces 2-methylbutanol.
A recombinant microorganism comprising at least one DNA molecule, wherein said at least one DNA molecule encodes at least two polypeptides that catalyze a substrate to product conversion selected from the group consisting of:
- a) L-threonine to 2-oxobutanate,
- b) 2-oxobutanate to 2-aceto-hydroxy-butyrate,
- c) 2-aceto-hydroxy-butyrate to 2,3-dihydroxy-3-methylvalerate, and
- d) 2,3-dihydroxy-3-methylvalerate to 2-keto-3-methylvalerate;
- and wherein said recombinant microorganism produces 2-methylbutanol.
A recombinant microorganism comprising at least one DNA molecule, wherein said at least one DNA molecule encodes (i) a polypeptide that catalyzes a 2-keto-3-methyl-valerate to 2-methylbutanal conversion, and (ii) a polypeptide that catalyzes a 2-methylbutanal to 2-methylbutanol conversion;
- and wherein said recombinant microorganism produces 2-methylbutanol.
A recombinant microorganism comprising at least one DNA molecule, wherein said at least one DNA molecule encodes at least two polypeptides that catalyze a substrate to product conversion selected from the group consisting of:
- (i) Pyruvate to citramalate,
- (ii) Citramalate to erythro-beta-methyl-D-malate, and
- (iii) erythro-beta-methyl-D-malate to 2-oxobutanoate;
- and wherein said recombinant microorganism produces 2-methylbutanol.
A composition comprising a compound of the formula I and/or II:
- wherein the compound comprises a fraction of modern carbon (fM 14C) of at least about 1.003.
A composition comprising a compound of the formula I, II, III, IV and/or V:
- wherein the compound comprises a fraction of modern carbon (fM 14C) of at least about 1.003.
Dependencies are inferred from the claim text.