# US 2009/0270602 A1 · Compositions comprising a linked acceptor moiety

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- Number: US 2009/0270602 A1
- Document ID: US20090270602A1
- Status: Published application
- Kind: A1
- Application number: 10/610,338
- Filed: 2003-06-30
- Published: 2009-10-29
- Earliest claimed: 1998-03-24
- Assignees: Boston Probes, Inc.
- Inventors: James M. Coull; Brian D. Gildea; Jens J. Hyldig-Nielsen
- Web page: https://patentgrep.com/patent/US20090270602A1

## CPC

- C12Q 1/6851: Quantitative amplification
- C07K 14/003: {Peptide-nucleic acids (PNAs)}
- C12Q 1/6818: involving interaction of two or more labels, e.g. resonant energy transfer
- C12Q 1/686: Polymerase chain reaction [PCR]
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

This invention is directed to compositions comprising a linked acceptor moiety.

## Claims

### Claim 1

A non-nucleic acid polymer bearing at least one linked acceptor moiety but not a linked donor moiety.

### Claim 2 (depends on 1)

The non-nucleic acid polymer of claim 1, wherein the polymer is a PNA.

### Claim 3 (depends on 2)

The non-nucleic acid polymer of claim 2, wherein PNA subunits of the polymer have the formula:
wherein,
each J is the same or different and is selected from the group consisting of: H, R1, OR1, SR1, NHR1, NR12, F, Cl, Br and I;
each K is the same or different and is selected from the group consisting of: O, S, NH and NR1;
each R1 is the same or different and is an alkyl group having one to five carbon atoms which may optionally contain a heteroatom or a substituted or unsubstituted aryl group;
each A is selected from the group consisting of a single bond, a group of the formula; —(CJ2)n- and a group of the formula; —(CJ2)sC(O)— wherein, J is defined above and each s is an integer from one to five;
each t is 1 or 2;
each u is 1 or 2; and
each L is the same or different and is independently selected from the group consisting of J, adenine, cytosine, guanine, thymine, uridine, 5-methylcytosine, 2-aminopurine, 2-amino-6-chloropurine, 2,6-diaminopurine, hypoxanthine, pseudoisocytosine, 2-thiouracil, 2-thiothymidine, other naturally occurring nucleobase analogs, other non-naturally occurring nucleobases, substituted and unsubstituted aromatic moieties, biotin, fluorescein and dabcyl.

### Claim 4 (depends on 3)

The non-nucleic acid polymer of claim 3, wherein each PNA subunit consists of a naturally occurring nucleobase attached to the aza nitrogen of a N-[2-(aminoethyl)]glycine backbone through a methylene carbonyl linkage.

### Claim 5 (depends on 1)

The non-nucleic acid polymer of claim 1, wherein the acceptor moiety is dabcyl.

### Claim 6 (depends on 5)

The non-nucleic acid polymer of claim 5, wherein the quencher moiety is dabcyl.

### Claim 7 (depends on 2)

The non-nucleic acid polymer of claim 2, wherein the acceptor moiety is dabcyl.

### Claim 8 (depends on 7)

The non-nucleic acid polymer of claim 7, wherein the quencher moiety is dabcyl.