Glutamate receptors in nutrient sensing, metabolism, Growth and Development
AssigneeThe George Washington University
InventorsSIVASUBRAMANIAN BALASUBRAMANIAN, FRANK J. TURANO
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Abstract
The present invention relates to methods that may be used to improve or modify nutrient sensing, absorption, metabolism, root growth, stomatal conductance, N use efficiency, C and N metabolism, plant biomass production and seed yield. More specifically, this invention is related to the glutamate receptors (GLRs) and their role(s) in nutrient sensing, metabolism, regulation of growth, development, and yield.
Claims43
Glutamate receptors of the sequences SEQ ID:1 and/or SEQ ID:2, wherein they regulate amino acid sensing, metabolism, growth and development.
A method for developing plant or cell line or clone that are efficient in N use, metabolism, growth and yield, comprising:
- obtaining and/or selecting a plant or cell line or clone that have modified levels or efficiency of glutamate receptor(s) compared to a progenitor plant.
A transgenic plant having a gene construct comprising a gene encoding a glutamate receptor operably linked to a plant promoter so that the glutamate receptor is overexpressed in the transgenic plant, and the transgenic plant exhibits:
- i) faster rate of growth;
- ii) greater fresh or dry weight at maturation;
- iii) greater fresh or dry weight at maturation;
- iv) greater fruit or seed yield;
- v) greater total plant nitrogen content;
- vi) greater fruit or seed nitrogen content;
- vii) greater free amino acid content in the whole plant;
- viii) greater free amino acid content in the fruit or seed;
- ix) greater protein content in seed or fruit;
- or
- x) greater protein content in a vegetative tissue;
- than a progenitor plant which does not contain the gene construct, when the transgenic plant and the progenitor plant are cultivated under identical growth conditions, wherein the glutamate receptor gene product is substantially identical to AtGLR3.2 polypeptide (SEQ ID NO:4).
An isolated nucleic acid comprising the mRNA of SEQ ID NO:
- 2.
An isolated polypeptide comprising the polypeptide of SEQ ID NO:
- 4.
Dependencies are inferred from the claim text.