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AssigneeNational Research Council of Canada
InventorsKeat (Thomas) H. Teoh, Darwin W. Reed, Devin R. Polichuk, Patrick S. Covello
Estimating the term…
Isolated nucleic acid molecules cloned from Artemisia annua encode artemisinic aldehyde double bond reductase and artemisinic/dihydroartemisinic aldehyde dehydrogenase. Artemisinic aldehyde double bond reductase enzymatically reduces artemisinic aldehyde to dihydroartemisinic aldehyde. Artemisinic/dihydroartemisinic aldehyde dehydrogenase enzymatically oxidizes dihydroartemisinic aldehyde to dihydroartemisinic acid and artemisinic aldehyde to artemisinic acid. The nucleic acid molecules, and the enzymes encoded thereby, may be used in processes to produce dihydroartemsinic aldehyde, dihydroartemisinic acid or artemisinic acid in a host cell. Dihydroartemisinic acid is a late precursor to the a antimalarial compound artemisinin.
Isolated nucleic acid molecule.comprising a nucleotide sequence having at least 70% nucleotide sequence identity to SEQ ID No.:
Isolated nucleic acid molecule comprising a nucleotide sequence encoding an artemisinic aldehyde double bond reductase having an amino acid sequence with at least 70% amino acid sequence identity to SEQ ID No.:
Isolated nucleic acid molecule comprising a nucleotide sequence having at least 70% nucleotide sequence identity to SEQ ID No.:
Isolated nucleic acid molecule comprising a nucleotide sequence encoding an artemisinic/dihydroartemisinic aldehyde dehydrogenase having an amino acid sequence with at least 70% amino acid sequence identity to SEQ ID No.:
Purified artemisinic aldehyde double bond reductase having an amino acid sequence with at least 70% amino acid sequence identity to SEQ ID No.:
Purified artemisinic/dihydroartemisinic aldehyde dehydrogenase having an amino acid sequence with at least 70% amino acid sequence identity to SEQ ID No.:
12-17. (canceled)
A method of selecting or developing plants with altered dihydroartemisinic aldehyde, dihydroartemisinic acid, artemisinic acid and/or artemisinin levels in a population of plants that naturally produces dihydroartemisinic aldehyde, dihydroartemisinic acid, artemisinic acid and/or artemisinin comprising:
Dependencies are inferred from the claim text.