Downy mildew resistant cucumber plants
AssigneeSeminis Vegetable Seeds, Inc.
InventorsNischit Shetty, Henk van Kooten, Bruno Sipeyre, Abert Grit, Joseph King, Marie Gretenkort, Inge Peck, M. Yolanda Duran
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Abstract
The present invention relates to cucumber lines having increased resistance to Downy Mildew. The present invention also relates to parts of cucumber plants from lines having increased Downy Mildew resistance, including seeds capable of growing cucumber plants with increased Downy Mildew resistance. Further provided are methods of producing such plants by genetic marker assisted selection.
Claims37
A method of producing a cucumber plant having resistance to Downy Mildew comprising the steps of:
- (a) crossing a cucumber plant of accession PI197088 with a second cucumber plant having at least one desired trait;
- and
- (b) selecting at least a first progeny cucumber plant resulting from the crossing that comprises resistance to Downy Mildew and the desired trait.
A seed of a cucumber line selected from the group consisting of ASL147-2027, EUR154-1012GY, EUR154-1021GY, GSP33-1094GY, GPN33-1093GY, 03/8020-20_TUP03_DMFL—1, 03/8024-19_TUP03_DMFL—1, and 03/8039-5_TUP03_DMFL—1, a sample of seed of said line having been deposited under ATCC Accession Number PTA-9375, ATCC Accession Number PTA-8930, ATCC Accession Number PTA-8931, ATCC Accession Number PTA-8953, ATCC Accession Number PTA-8954, ATCC Accession Number ______, ATCC Accession Number ______, and ATCC Accession Number ______, respectively.
A tissue culture of regenerable cells of a cucumber line selected from the group consisting of ASL147-2027, EUR154-1012GY, EUR154-1021GY, GSP33-1094GY, GPN33-1093GY, 03/8020-20_TUP03_DMFL—1, 03/8024-19_TUP03_DMFL—1, and 03/8039-5_TUP03_DMFL—1, a sample of seed of said line having been deposited under ATCC Accession Number PTA-9375, ATCC Accession Number PTA-8930, ATCC Accession Number PTA-8931, ATCC Accession Number PTA-8953, ATCC Accession Number PTA-8954, ATCC Accession Number ______, ATCC Accession Number ______, and ATCC Accession Number ______, respectively.
A method for producing a seed of a line ASL147-2027, EUR154-1012GY, EUR154-1021GY, GSP33-1094GY, GPN33-1093GY, 03/8020-20_TUP03_DMFL—1, 03/8024-19_TUP03_DMFL—1, or 03/8039-5_TUP03_DMFL—1 cucumber plant comprising the steps of:
- (a) crossing a cucumber plant of a line selected from the group consisting of ASL147-2027, EUR154-1012GY, EUR154-1021GY, GSP33-1094GY, GPN33-1093GY, 03/8020-20_TUP03_DMFL—1, 03/8024-19_TUP03_DMFL—1, and 03/8039-5_TUP03_DMFL—1, a sample of seed of said line having been deposited under ATCC Accession Number PTA-9375, ATCC Accession Number PTA-8930, ATCC Accession Number PTA-8931, ATCC Accession Number PTA-8953, ATCC Accession Number PTA-8954, ATCC Accession Number ______, ATCC Accession Number ______, and ATCC Accession Number ______, respectively, with a second cucumber plant;
- and
- (b) allowing seed of a line ASL147-2027, EUR154-1012GY, EUR154-1021GY, GSP33-1094GY, GPN33-1093GY, 03/8020-20_TUP03_DMFL—1, 03/8024-19_TUP03_DMFL—1, or 03/8039-5_TUP03_DMFL—1-derived cucumber plant to form.
A method of introducing a desired trait into a cucumber line selected from the group consisting of ASL147-2027, EUR154-1012GY, EUR154-1021GY, GSP33-1094GY, GPN33-1093GY, 03/8020-20_TUP03_DMFL—1, 03/8024-19_TUP03_DMFL—1, and 03/8039-5_TUP03_DMFL—1, comprising:
- (a) crossing a plant of a line selected from the group consisting of ASL147-2027, EUR154-1012GY, EUR154-1021GY, GSP33-1094GY, GPN33-1093GY, 03/8020-20_TUP03_DMFL—1, 03/8024-19_TUP03_DMFL—1, and 03/8039-5_TUP03_DMFL—1, a sample of seed of said line having been deposited under ATCC Accession Number PTA-9375, ATCC Accession Number PTA-8930, ATCC Accession Number PTA-8931, ATCC Accession Number PTA-8953, ATCC Accession Number PTA-8954, ATCC Accession Number ______, ATCC Accession Number ______, and ATCC Accession Number ______, with a second cucumber plant that comprises a desired trait to produce F1 progeny;
- (b) selecting an F1 progeny that comprises the desired trait;
- (c) crossing the selected F1 progeny with a plant of a line selected from the group consisting of ASL147-2027, EUR154-1012GY, EUR154-1021GY, GSP33-1094GY, GPN33-1093GY, 03/8020-20_TUP03_DMFL—1, 03/8024-19_TUP03_DMFL—1, and 03/8039-5_TUP03_DMFL—1, a sample of seed of said line having been deposited under ATCC Accession Number PTA-9375, ATCC Accession Number PTA-8930, ATCC Accession Number PTA-8931, ATCC Accession Number PTA-8953, ATCC Accession Number PTA-8954, ATCC Accession Number ______, ATCC Accession Number ______, and ATCC Accession Number ______, respectively, to produce backcross progeny;
- (d) selecting backcross progeny comprising the desired trait and the physiological and morphological characteristic of a cucumber line selected from the group consisting of ASL147-2027, EUR154-1012GY, EUR154-1021GY, GSP33-1094GY, GPN33-1093GY, 03/8020-20_TUP03_DMFL—1, 03/8024-19_TUP03_DMFL—1, and 03/8039-5_TUP03_DMFL—1;
- and
- (e) repeating steps (c) and (d) three or more times in succession to produce selected fourth or higher backcross progeny that comprise the desired trait.
A method of producing a plant of a cucumber line selected from the group consisting of ASL147-2027, EUR154-1012GY, EUR154-1021GY, GSP33-1094GY, GPN33-1093GY, 03/8020-20_TUP03_DMFL—1, 03/8024-19_TUP03_DMFL—1, and 03/8039-5_TUP03_DMFL—1, a sample of seed of said line having been deposited under ATCC Accession Number PTA-9375, ATCC Accession Number PTA-8930, ATCC Accession Number PTA-8931, ATCC Accession Number PTA-8953, ATCC Accession Number PTA-8954, ATCC Accession Number ______, ATCC Accession Number ______, and ATCC Accession Number ______, respectively, comprising an added desired trait, the method comprising introducing a transgene conferring the desired trait into a plant of cucumber a line selected from the group consisting of ASL147-2027, EUR154-1012GY, EUR154-1021GY, GSP33-1094GY, GPN33-1093GY, 03/8020-20_TUP03_DMFL—1, 03/8024-19_TUP03_DMFL—1, and 03/8039-5_TUP03_DMFL—1.
A plant of a cucumber line that exhibits one or more traits selected from the group consisting of:
- resistance to Angular leaf spot, Anthracnose race 1, Cucumber Scab, Downy mildew, Powdery Mildew, Papaya Ringspot, Zucchini Yellow Mosaic, Cucumber Mosaic Virus, and fruit quality acceptable to market, wherein the combination of traits is controlled by genetic means for the expression of such one or more traits found in a cucumber line selected from the group consisting of:
- ASL147-2027, EUR154-1012GY, EUR154-1021GY, GSP33-1094GY, GPN33-1093GY, 03/8020-20_TUP03_DMFL—1, 03/8024-19_TUP03_DMFL—1, and 03/8039-5_TUP03_DMFL—1, a sample of seed of said line having been deposited under ATCC Accession Number PTA-9375, ATCC Accession Number PTA-8930, ATCC Accession Number PTA-8931, ATCC Accession Number PTA-8953, ATCC Accession Number PTA-8954, ATCC Accession Number ______, ATCC Accession Number ______, and ATCC Accession Number ______.
Dependencies are inferred from the claim text.