Adenovirus 36 E4 orf 1 Gene and Protein and Their Uses
AssigneesWayne State Univeristy, Board of Sup. of LSU and Agr. & Mechanical College
InventorsNikhil V. Dhurandhar, Thomas C. Holland, Zhong Q. Wang
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Abstract
Expression of the E4 orf 1 gene of Ad-36 alone has been discovered to be responsible for the increased insulin sensitivity observed in Ad-36 infected animals, including increased adipogenesis. Ad-36 E4 orf 1 protein can be used to increase insulin sensitivity and ameliorate diabetes. Additionally, drugs that mimic the action of Ad-36 E4 orf 1 protein could be found. Ad-36 E4 orf 1 could also be used to increase fat cells in lipodystrophy. We have also discovered that Ad-36 infection in human skeletal muscle cells increased differentiation and insulin independent glucose uptake. It is expected that infection with Ad-36 E4 orf 1 gene will also cause these effects.
Claims31
An isolated nucleic acid sequence comprising SEQ ID NO:
- 1, or comprising oligonucleotides that encodes the same amino acid sequence in accordance with the degeneracy of the genetic code.
An isolated protein whose amino acid sequence comprises SEQ ID NO:
- 2.
A method of treating or ameliorating the symptoms of a disease in a mammalian patient wherein the disease is chosen from the group consisting of lipodystrophy, diabetes, and insulin resistance, said method comprising administering to the patient a therapeutically effective amount of Adenovirus-36 E4 orf 1 protein, wherein the patient's symptoms improve following said administering.
A method for predicting insulin sensitivity in a patient, said method comprising assaying a tissue from the patient for the presence of one or more compounds selected from the group consisting of Adenovirus-36 E4 orf 1 DNA, Adenovirus-36 E4 orf 1 mRNA, and Adenovirus-36 E4 orf 1 protein, wherein the presence of said compound indicates a patient with a higher insulin sensitivity than a patient without said compound.
Dependencies are inferred from the claim text.