Biosensor solid substrate with integrated temperature control and a method to make the same
AssigneeKoninklijke Philips Electronics N.V.
InventorsDirk Jan Broer, Roel Penterman, Ralph Kurt, Emiel Peeters, Hendrik Roelof Stapert, Anke Pierik, Aleksey Kolesnychenko, Hendrik De Koning
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Abstract
This invention provides a biosensor solid or hydrogel substrate comprising one or more temperature indicating agents, each of said one or more temperature indicating agents operating by changing its optical properties and being deposited in and/or at the surface of the biosensor solid or hydrogel substrate as one or more layers and/or spots
Claims28
A non-porous or porous biosensor substrate made of solid or hydrogel material comprising one or more temperature indicating agents, each of said one or more temperature indicating agents operating by changing its optical properties and being deposited in and/or at the surface of the biosensor solid or hydrogel substrate as one or more layers and/or spots.
A biosensor device comprising
- a chamber (1) including a porous or non-porous biosensor substrate (2) made of a solid or hydrogel material, said biosensor substrate (2) comprising one or more temperature indicating agents (9) operating by changing their optical properties,
- inlet means (3) for introducing a sample fluid (4) suspected to contain one or more target biological compounds into said chamber (1) in such a way that said sample fluid (4) contacts said biosensor substrate (2),
- means (5) for analyzing said biosensor substrate (2) after said sample fluid (4) having contacted said biosensor substrate (2) so as to determine the presence and/or the concentration of said one or more target biological compounds onto said biosensor substrate, and
- means (6) for analyzing said biosensor substrate (2) so as to retrieve temperature-related information from said one or more temperature indicating agents (9).
A method of analysis of a sample fluid suspected of containing one or more target biological compounds comprising the steps of:
- a) analyzing a porous or non-porous biosensor substrate made of a solid or hydrogel material and comprising one or more temperature indicating agents, said one or more temperature indicating agents operating by changing their optical properties, to gain temperature-related information from said one or more temperature indicating agent,
- b) contacting said sample fluid with said biosensor substrate, and
- c) analyzing said biosensor substrate after contacting said sample fluid so as to determine the presence and/or the concentration of said one or more target biological compounds.
A device for performing a PCR, said device comprising:
- A recipient for receiving the biological molecular species to be amplified, said recipient comprising a hydrogel, said hydrogel being temperature responsive or comprising one or more temperature indicating agents,
- Heating and/or cooling means, and
- means for analyzing said hydrogel so as to retrieve temperature-related information from said one or more temperature indicating agents.
Dependencies are inferred from the claim text.