Method for regulating agrobacterium-mediated transformation
AssigneesCsir, Pioneer Hi-Bred International, Inc.
InventorsLuke MEHLO, Zuo-Yu Zhao
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Abstract
A method for regulating expression of a virulence gene of Agrobacterium is described. The method comprises the steps of stimulating cereal cells, such as sorghum, so as to produce an active, typically phenolic, compound and exposing the Agrobacterium to this compound. The compound induces expression of the virulence gene of the Agrobacterium, effecting T-DNA transfer from the Agrobacterium to the cereal cells, which are thereby transformed.
Claims21
1-42. (canceled)
A method for regulating expression of at least one virulence gene of Agrobacterium, the method comprising the steps of:
- stimulating cereal cells to produce at least one active compound;
- and
- exposing the Agrobacterium to the active compound(s).
An Agrobacterium-mediated method for transforming plant cereal cells, the method comprising:
- (i) stimulating cereal cells to produce at least one active compound;
- and
- (ii) exposing the Agrobacterium to the cereal cells and the active compound(s) produced by the cereal cells, such that the active compound induces expression of at least one virulence gene of the Agrobacterium and effects T-DNA transfer from the Agrobacterium to the cereal cells, thereby transforming the cereal cells.
Dependencies are inferred from the claim text.