Mass spectrometric analysis method
AssigneeEidgenossische Technische Hochschule Zurich
InventorsAlexis Nazabal, Ryan Wenzel, Renato Zenobi
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Abstract
Methods of using mass spectrometry and in particular matrix assisted laser Ligand-Complex from Target and desorption-ionization (MALDI) mass spectrometry to analyze, or otherwise detect the presence of or determine the identity of intact ions of undigested, unfragmented covalently stabilized supramolecular target-ligand-complexes, as well as the use of these methods in various biological application such as characterization of antibodies, drug discovery, and complexomics including automated or higher throughput applications.
Claims11
1-11. (canceled)
Method for analyzing intact ions of undigested, unfragmented supramolecular target-ligand-complexes using mass spectrometry comprising the steps of:
- a) contacting a non-covalently bonded, supramolecular target-ligand-complex with a crosslinking reagent to form a covalently stabilized supramolecular target-ligand-complex, and
- b) analyzing the intact ions from the undigested, unfragmented covalently stabilized supramolecular target-ligand-complex by mass spectrometry.
Method for detecting intact ions of an undigested, unfragmented supramolecular target-ligand-complex using mass spectrometry comprising:
- (a) obtaining a first sample comprising a non-covalently bonded supramolecular target-ligand-complex, (b) contacting said first sample with a crosslinking reagent to obtain a second sample comprising a covalently stabilized supramolecular target-ligand-complex;
- (c) mixing said second sample with a matrix solution to obtain a sample/matrix mixture, (d) depositing said sample/matrix solution on a substrate, thereby forming a homogeneous, thin layer;
- (e) illuminating the substrate with radiation from a laser whereby said covalently stabilized supramolecular target-ligand-complex in the sample/matrix mixture is desorbed and intact ions are generated;
- and (f) mass separating and detecting said intact ions of the undigested, unfragmented covalently stabilized supramolecular target-ligand-complex using a mass separation and analysis format.
Dependencies are inferred from the claim text.