Use Polysaccharides for Promotion of Enzymatic Activity
AssigneeGenzyme Corporation
InventorsFrank Riske, Michael Hayes, Gary Lazarus
Life of this patent
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Abstract
This disclosure provides methods and compositions for the promotion of enzymatic activity of Target Enzymes, including but not limited to oligosaccharide/polysaccharide enzymes, protein enzymes, polynucleotide enzymes. The methods involve use of a non-naturally occurring polysaccharide (including but not limited HES) for promoting the enzymatic activity of an enzyme in liquid milieu, wherein the concentration of the polysaccharide in the composition comprising the Target Enzyme is from about 0.01% to about 55% w/v.
Claims34
A composition comprising:
- (a) a Target Enzyme selected from the group consisting of:
- an oligosaccharide/polysaccharide enzyme, a protein enzyme, a polynucleotide enzyme, and a lipase, and;
- (b) a non-naturally occurring starch,
- wherein the composition comprises from about 0.01% to about 55% w/v of the polysaccharide and wherein the activity of the Target Enzyme has been promoted by the non-naturally occurring polysaccharide.
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A composition comprising:
- (a) a Target Enzyme selected from the group consisting of:
- an oligosaccharide/polysaccharide enzyme, a protein enzyme, a polynucleotide enzyme, and a lipase, and;
- (b) a non-naturally occurring starch,
- wherein the composition comprises from about 1% to about 50% w/v of the polysaccharide.
A pharmaceutical composition comprising:
- (a) a Target Enzyme which is a therapeutic protein and;
- (b) a non-naturally occurring starch,
- wherein the composition comprises from about 0.01% to about 55% w/v of the polysaccharide.
A composition comprising:
- (a) an oligosaccharide/polysaccharide enzyme;
- (b) between about 1% and about 12% w/v HES;
- and
- (c) a substrate for the oligosaccharide/polysaccharide enzyme.
A method of promoting enzymatic activity of a Target Enzyme comprising:
- (a) combining a Target Enzyme with about 0.01% to about 55% w/v of a non-naturally occurring starch, thereby producing a combination;
- and
- (b) maintaining the combination under conditions sufficient to promote the enzymatic activity of the Target Enzyme.
A method of promoting the enzymatic activity of sialidase, β-galactosidase, and β-hexosaminidase comprising:
- (a) combining the sialidase, β-galactosidase, and β-hexosaminidase and between about 1% and about 12% w/v HES, thereby producing a combination;
- and
- (b) maintaining the combination under conditions sufficient to promote the enzymatic activity of the sialidase, β-galactosidase, and β-hexosaminidase.
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Dependencies are inferred from the claim text.