Neuronal progenitors from feeder-free human embryonic stem cell culture
AssigneeUniversity of Georgia Research Foundation, Inc.
InventorsSteve Stice, Soojung Shin, Sujoy Dhara
Life of this patent
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Abstract
The present invention relates to methods for producing feeder cell-free neuroprogenitor cells (preferably adherent) from embryonic stems cells, preferably human embryonic stem cells, the feeder cell-free neuroprogenitor cells, preferably human cells themselves, as well as methods for producing feeder cell-free samples of neuronal cells, preferably adherent human neuronal cells and the feeder cell-free neuronal cells themselves. Pharmaceutical compositions and methods of treating neurodegenerative diseases as well as the use of the described cells in assay systems is also described.
Claims7
A method for producing feeder cell free neuroprogenitor cells, said method comprising the steps of:
- a. Contacting adherent primate embryonic stem cells in a serum free differentiation medium comprising the components of a minimum essential medium and basic fibroblast growth factor and optionally, non-essential amino acids, leukaemia inhibitory factor (LIF), an antibiotic, mercaptoethanol or another cell medium selected from B27, N2 or mixtures thereof, with an effective amount of at least one embryonic stem cell differentiation protein to differentiate said adherent primate embryonic stem cells in to neuroprogenitor cells without embryoid body formation;
- and
- b. Passaging said neuroprogenitor cells.
2-30. (canceled)
Dependencies are inferred from the claim text.