Cell labeling with perfluorocarbon nanoparticles for magnetic resonance imaging and spectroscopy
AssigneeWashington University
InventorsSamuel A. Wickline, Gregory M. Lanza
Life of this patent
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Abstract
Methods of obtaining cells internally labeled with perfluorocarbon nanoparticles suitable for magnetic resonance imaging and spectroscopy are disclosed. Also disclosed are methods for obtaining magnetic resonance imaging data from labeled under clinically relevant scan times and field strengths. Finally, the application further discloses methods of specifically detecting and distinguishing magnetic resonance imaging and spectroscopy data from two distinct sets of cells labeled with distinct types of perfluorocarbon nanoparticles.
Claims37
A method for obtaining an endothelial precursor cell suitable for magnetic resonance imaging comprising the steps of:
- a. providing an endothelial precursor cell;
- b. incubating said endothelial precursor cell in a cell culture media containing a plurality of perfluorocarbon nanoparticles for a period of time and at a perfluorocarbon nanoparticle concentration sufficient to result in internalization of a detectable level of perfluorocarbon nanoparticles;
- and
- c. separating said endothelial precursor cell from step (b) from said culture media containing perfluorocarbon nanoparticles;
- thereby obtaining an endothelial precursor cell suitable for magnetic resonance imaging.
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A method of obtaining two distinct magnetic resonance imaging data sets derived from two distinct cells introduced into a system, comprising the steps of:
- a) obtaining a first cell containing a first intracellular perfluorocarbon nanoparticle, wherein said first intracellular perfluorocarbon nanoparticle comprises a perfluoro-15-crown-5-ether core component and wherein said first intracellular perfluorocarbon nanoparticle is at a detectable level in said first cell;
- b) obtaining a second cell containing a second intracellular perfluorocarbon nanoparticle, wherein said second intracellular perfluorocarbon nanoparticle comprises a perfluorooctylbromide core component and wherein said second intracellular perfluorocarbon nanoparticle is at a detectable level in said second cell;
- c) introducing said first cell from step (a) and said second cell from step (b) into a system;
- d) exposing said system from step (c) to a first magnetic field and obtaining magnetic resonance imaging data for said first cell with a magnetic resonance imaging method that specifically detects a first 19F MRI signal from said perfluoro-15-crown-5-ether core component to obtain a first imaging data set from said first cell,
- e) exposing said system from step (c) to a second magnetic field and obtaining magnetic resonance imaging data for said second cell with a magnetic resonance imaging method that specifically detects a second 19F MRI signal from said perfluorooctylbromide core component to obtain a second imaging data set from said second cell, thereby obtaining two distinct magnetic resonance imaging data sets derived from two distinct cells introduced into a system.
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A method of obtaining two distinct magnetic resonance spectroscopy data sets derived from two distinct cells introduced into a system, comprising the steps of:
- a) obtaining a first cell containing a first intracellular perfluorocarbon nanoparticle, wherein said first intracellular perfluorocarbon nanoparticle comprises a perfluoro-15-crown-5-ether core component and wherein said first intracellular perfluorocarbon nanoparticle is at a detectable level of said first cell;
- b) obtaining a second cell containing a second intracellular perfluorocarbon nanoparticle, wherein said second intracellular perfluorocarbon nanoparticle comprises a perfluorooctylbromide core component and wherein said second intracellular perfluorocarbon nanoparticle is at a detectable level in said second cell;
- c) introducing said first cell from step (a) and said second cell from step (b) into a system;
- d) exposing said system from step (c) to a first magnetic field and obtaining magnetic resonance spectroscopy data for said first cell with a magnetic resonance spectroscopy method that specifically detects a first 19F MRI signal from said perfluoro-15-crown-5-ether core component to obtain a first spectroscopy data set from said first cell,
- e) exposing said system from step (c) to a second magnetic field and obtaining magnetic resonance spectroscopy data for said second cell with a magnetic resonance spectroscopy method that specifically detects a second 19F MRI signal from said perfluorooctylbromide core component to obtain a second spectroscopy data set from said second cell, thereby obtaining two distinct magnetic resonance spectroscopy data sets derived from two distinct cells introduced into a system.
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A method for obtaining a cell suitable for magnetic resonance imaging comprising the steps of:
- a. providing at least one cell;
- b. treating said cell or cells in a cell culture media containing a plurality of non-targeted perfluorocarbon nanoparticles with ultrasound energy for a period of time and at a perfluorocarbon nanoparticle concentration sufficient to result in internalization of a detectable level of perfluorocarbon nanoparticles;
- and
- c. separating said cell from step (b) from said culture media containing perfluorocarbon nanoparticles;
- thereby obtaining a cell suitable for magnetic resonance imaging.
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Dependencies are inferred from the claim text.