# US 2009/0260995 A1 · Polymer based biosensor

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- Number: US 2009/0260995 A1
- Document ID: US20090260995A1
- Status: Published application
- Kind: A1
- Application number: 12/382,604
- Filed: 2009-03-19
- Published: 2009-10-22
- Earliest claimed: 2008-04-16
- Inventors: Keith Warriner; Asadeh Namvar
- Web page: https://patentgrep.com/patent/US20090260995A1

## CPC

- G01N 33/56961: {Plant cells or fungi}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A biosensor comprising a conducting polymer and a facilitating agent that combine to form a polymeric matrix that interacts with electrophilic compounds, wherein an electrochemical property of the polymer is altered in the presence of an electrophilic compound.

## Claims

### Claim 1

A biosensor for detecting one or more electrophilic compounds, comprising a conducting polymer and a facilitating agent that combine to form a polymeric matrix that interacts with electrophilic compounds, wherein the interaction with an electrophilic compound induces a change in an electrochemical property of the polymer.

### Claim 2 (depends on 1)

A biosensor as defined in claim 1, wherein the conducting polymer is selected from the group consisting of polypyrrole, polyaniline, polythiophene, polyacetylene, and derivatives thereof.

### Claim 3 (depends on 2)

A biosensor as defined in claim 2, wherein the facilitating agent is selected from the group consisting of patulin and derivatives thereof, compounds having a molecular weight >150, pyrrole derivatives, nucleophilic compounds that react with an electrophilic compound to be detected, nucleic acids, proteins, cysteine and tyrosine.

### Claim 4 (depends on 3)

A biosensor as defined in claim 3, wherein the facilitating agent is patulin.

### Claim 5 (depends on 1)

A biosensor as defined in claim 1, wherein the facilitating agent is bovine serum albumin.

### Claim 6 (depends on 1)

A biosensor as defined in claim 1, wherein the facilitating agent is DNA.

### Claim 7

A biosensor as defined in claiml, wherein the electrochemical property is conductivity.

### Claim 8 (depends on 1)

A biosensor as defined in claim 1, wherein the electrophilic compound is a mycotoxin.

### Claim 9 (depends on 8)

A biosensor as defined in claim 8, wherein the mycotoxin is selected from the group consisting of an aflatoxin, ochratoxin A, patulin and a fusarium toxin.

### Claim 10

A method of detecting a target electrophilic compound in a sample comprising the steps of:
1) contacting the sample with a biosensor comprising a conducting polymer and a facilitating agent that combine to form a polymeric matrix that interacts with electrophilic compounds to induce a change in an electrochemical property of the polymer; and
2) determining whether an electrochemical property of the polymer is altered, wherein a change in an electrochemical property of the polymer is indicative of the presence of an electrophilic compound in the sample.

### Claim 11 (depends on 10)

A method as defined in claim 10, wherein the biosensor comprises a conducting polymer selected from the group consisting of polypyrrole, polyaniline, polythiophene, polyacetylene, and derivatives thereof.

### Claim 12 (depends on 10)

A method as defined in claim 10, wherein the biosensor comprises a facilitating agent selected from the group consisting of patulin and derivatives thereof, compounds having a molecular weight >150, pyrrole derivatives, nucleophilic compounds that react with an electrophilic compound to be detected, nucleic acids, proteins, cysteine and tyrosine.

### Claim 13 (depends on 10)

A method as defined in claim 10, wherein the electrochemical property is conductivity.

### Claim 14 (depends on 13)

A method as defined in claim 13, wherein a decrease in conductivity is indicative of the presence of an electrophilic compound in the sample.

### Claim 15 (depends on 10)

A method as defined in claim 10, wherein the electrophilic compound is a mycotoxin.

### Claim 16 (depends on 15)

A method as defined in claim 15, wherein the mycotoxin is selected from the group consisting of an aflatoxin, ochratoxin A, patulin and a fusarium toxin.

### Claim 17 (depends on 10)

A method as defined in claim 10, wherein the change in the electrochemical property of the polymer is proportional to the amount of the electrophilic compound in the sample.

### Claim 18 (depends on 10)

A method as defined in claim 10, for detecting an electrophilic compound at a limit of less than 100 ppb.

### Claim 19 (depends on 1)

A biosensor as defined in claim 1, wherein the interaction results in the formation of an adduct.