Compositions and methods for diagnosis and treatment of chronic inflammatory diseases
AssigneeThe George Washington University
InventorsMICHAEL BUKRINSKY, STEPHANIE CONSTANT
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Abstract
This invention relates to methods and compositions for diagnosis and treatment of chronic inflammatory diseases by blocking CD147 interaction with extracellular cyclophilin. Specifically, the methods and compositions of this invention regulate recruitment of leukocyte to the infection site by specifically blocking the CD147 domain involved with the chemotactic function without blocking the CD147 domain involved with EMMPRIN function.
Claims20
A method for treatment or amelioration of one or more symptoms of a chronic inflammatory disease in an individual in need thereof comprising, administering a therapeutically effective amount of a compound that blocks CD147 interaction with an extracellular cyclophilin without blocking the CD147 domain involved with EMMPRIN function, wherein the compound regulates recruitment of leukocyte to a target tissue and thereby treat or ameliorate symptoms of the chronic inflammatory disease.
A drug screening method for selecting compounds that inhibit binding between CD147 and external cyclophilins without blocking the CD147 domain involved with EMMPRIN comprising, contacting the CD147 or cyclophilin polypeptide or a variant thereof with a library of compounds suspected of having antagonist or agonist activity with CD147 and/or cyclophilins, identifying select compounds that binds to otherwise interacts CD147 and/or cyclophilins, and assaying the biological activity of the select compounds, wherein the selected compound reduces or inhibits recruitment of leukocyte to a target tissue.
A pharmaceutical composition for treatment of a chronic inflammatory disease in an individual in need thereof comprising, a therapeutically effective amount of a compound that blocks binding between CD147 and an extracellular cyclophilin without blocking the CD147 domain involved with EMMPRIN function, and a suitable carrier or diluent.
A diagnostic test kit for to diagnose, detect or quantify an inflammatory disease in a subject in need thereof, comprising a compound that blocks binding between CD147 and an extracellular cyclophilin without blocking the CD147 domain involved with EMMPRIN function, inactive agents used for biological assays including buffers, minerals, and water, among others, instructions for the use of the compound and the biological assay to predict or diagnose the occurrence or recurrence of an inflammatory mediated disease in the subject.
Dependencies are inferred from the claim text.