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AssigneeHelmholtz-Zentrum FüR Infektionsforschung GmbH
InventorsManuel Ferrer, Kenneth Timmis, Tatjana Chernikova, Peter Golyshin, Michail Yakimov
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The invention relates to the growth temperature of organisms, especially plants and microorganisms and the manipulation of the tolerable cultivation temperature. More specifically, the present invention relates to the expression of heterologous proteins in microorganisms, and especially to the heterologous expression of heat sensitive proteins in bacteria, either gram-negative or gram-positive. In a first aspect, the present invention provides a method for manipulation of cells and the resultant cells, wherein at least one gene from a psychrophilic micro organism coding for at least one chaperone or chaperonin is expressed. Such cells are selected among cultivated eukaryotic cells, i.e. animal and plant cells and entire plants, gram-negative and gram-positive bacteria, fungi and yeasts.
1-20. (canceled)
Process for producing a protein by heterologous expression in a host microorganism containing a gene sequence encoding the heterologous protein, wherein the micro-organism is genetically manipulated to contain a DNA sequence encoding the chaperonin of Oleispira antarctica Cpn60 and/or Cpn10 (Seq ID No. 1 and/or 2) or a functional mutant thereof and in using a cultivation temperature of below 25° C.
Plant, wherein it can grow at lower ambient temperatures due to the presence of a DNA sequence encoding a cold active functional chaperonin of a psychrophilic bacterium or plant.
Dependencies are inferred from the claim text.