# US 2009/0253201 A1 · Embryonic stem cells capable of differentiating into desired cell lines

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- Number: US 2009/0253201 A1
- Document ID: US20090253201A1
- Status: Published application
- Kind: A1
- Application number: 12/456,092
- Filed: 2009-06-11
- Published: 2009-10-08
- Earliest claimed: 1993-11-03
- Assignees: Diacrin, Inc.
- Inventors: Jonathan H. Dinsmore; Judson Ratliff
- Web page: https://patentgrep.com/patent/US20090253201A1

## CPC

- C12N 5/0068: {General culture methods using substrates (for specific animal cell type C12N5/06)}
- C07K 14/475: Growth factors; Growth regulators
- C07K 14/715: for cytokines; for lymphokines; for interferons
- C12N 5/0619: {Neurons}
- G01N 33/5005: {involving human or animal cells (immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025)}
- C12N 2500/25: Insulin-transferrin; Insulin-transferrin-selenium
- C12N 2500/36: Lipids
- C12N 2500/84: from mammals
- C12N 2501/11: Epidermal growth factor [EGF]
- C12N 2501/18: Liver cell growth factor (LCGF, Gly-His-Lys)
- C12N 2501/36: Somatostatin
- C12N 2501/39: Steroid hormones
- C12N 2501/395: Thyroid hormones
- C12N 2501/91: Heparin
- C12N 2506/02: from embryonic cells
- C12N 2510/00: Genetically modified cells
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

An embryonic stem cell which may be induced to differentiate homogeneously into a desired primary cell line. The embryonic stem cell may be engineered with DNA, which encodes a protein or polypeptide which promotes differentiation of the stem cell into a specific cell line, such as, for example, a neuronal cell line, a muscle cell line, or a hematopoietic cell line. The DNA may encode a transcription factor found in the particular cell line. In another alternative, a desired cell line is produced from embryonic stem cells by culturing embryonic stem cells under conditions which provide for a three-dimensional network of embryonic stem cells, and then stimulating embryonic stem cells with an agent, such as retinoic acid, or dimethylsulfoxide, which promotes differentiation of the embryonic stem cells into the desired cell line, such as, for example, a neuronal cell line, or a muscle cell line.

## Claims

### Claim at position 1

1-29. (canceled)

### Claim 30

An embryonic stem cell, said embryonic stem cell having been genetically engineered with DNA which encodes MyoD, which promotes differentiation of said embryonic stem cell into a muscle cell.