Avipox recombinants expressing foot and mouth disease virus genes
OwnerRobert Nordgren, Sheena May Loosmore +2inventors
InventorsRobert Nordgren, Sheena May Loosmore, Jean-Christophe Francis Audonnet, Marvin J. Grubman
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Abstract
The present invention relates to modified poxyiral vectors and to methods of making and using the same. In particular, the invention relates to recombinant avipox that expresses gene products of foot and mouth disease virus (FMDV), and to compositions or vaccines that elicit immune responses directed to FMDV gene products and which can confer protective immunity against infection by FMDV.
Claims16
1-30. (canceled)
A method of producing a recombinant avipox vector comprising at least one nucleic acid molecule encoding one or more foot-and-mouth disease virus (FMDV) antigen(s), comprising the steps of:
- (a) linearizing a donor plasmid with a restriction endonuclease, wherein the donor plasmid comprises restriction endonuclease cleavage sites or a multiple cloning site;
- and
- (b) ligating at least one nucleic acid molecule comprising
- (i) a nucleic acid sequence encoding one or more FMDV antigen(s),
- (ii) a viral promoter sequence, and
- (iii) insertion sequences flanking (i) and (ii) that have complementary restriction endonuclease cleavage sites to the donor plasmid at FMDV antigens,
- thereby producing the recombinant avipox vector.
Dependencies are inferred from the claim text.