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US 2009/0253183 A1Published application
Amplicon Rescue Multiplex Polymerase Chain Reaction for Amplification of Multiple Targets
AssigneeHudsonAlpha Institute for Biotechnology
InventorJian Han
Life of this patent
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Abstract
Disclosed is a method for amplifying and detecting polynucleotides which can provide sensitive, specific detection of multiple targets from a clinical specimen within a relatively short time.
Claims8
A method comprising
- amplifying one or more target nucleic acids using high-concentration target-specific primers in a first amplification reaction, thereby producing at least one nucleic acid amplicon containing at least one common primer binding site;
- rescuing the at least one nucleic acid amplicon;
- and
- amplifying the at least one nucleic acid amplicon in a second amplification reaction using at least one common primer which binds to the at least one common primer binding site.
1 more element
A primer kit comprising primers chosen from the group consisting of SEQ ID NO:
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- 92, and combinations thereof.
89 more elements
Dependencies are inferred from the claim text.