Nucleotide sequences for regulating gene expression in plant trichomes and constructs and methods utilizing same
AssigneeEvogene Ltd.
InventorsGil Ronen, Larisa Rabinovich, Rafael Meissner, Hagai Karchi
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Abstract
Novel plant derived regulatory sequences and constructs and methods of using such sequences for directing expression of exogenous polynucleotide sequences in trichomes are provided.
Claims26
An isolated polynucleotide comprising a nucleic acid sequence at least 80% identical to SEQ ID NO:26 or 29, wherein said nucleic acid sequence is capable of regulating expression of at least one polynucleotide sequence operably linked thereto in trichomes.
An isolated polynucleotide comprising a nucleic acid sequence encoding a peptide capable of directing transport of a polypeptide fused thereto into a subcellular compartment of a trichome, wherein said peptide is encoded by the polynucleotide sequence set forth in SEQ ID NO:59, 61, 63, 65 or 75.
A method of producing a polypeptide of interest in plant trichomes, the method comprising:
- (a) expressing the polypeptide of interest in the plant trichomes;
- and
- (b) down-regulating a level of at least one molecule endogenous to the plant trichomes, said at least one molecule being capable of interfering with expression, accumulation or stability of the polypeptide of interest.
A method of producing a molecule of interest in plant trichomes, the method comprising:
- (a) expressing a polypeptide capable of directly or indirectly increasing a level of the molecule of interest in the plant trichomes;
- and
- (b) down-regulating a level of at least one molecule endogenous to the plant trichomes, said at least one molecule being capable of interfering with accumulation or stability of the molecule of interest, thereby producing the molecule in the plant trichomes.
A plant genetically modified to express a molecule of interest in trichomes, wherein said plant is further modified or selected capable of accumulating less than 50% of average volume of undesired compounds in trichome cells of said plant species.
A method of harvesting trichomes, exudates and/or content thereof, the method comprising:
- (a) incubating a trichome-containing plant tissue in a liquid such that trichome exudates and content is released into said liquid, wherein incubating is effected while avoiding friction of said trichome-containing plant tissue with a solid surface;
- and
- (b) collecting said liquid, to thereby harvest the trichome exudates and/or content.
An apparatus for mechanical harvesting of trichome exudates and/or content, the apparatus comprising a mechanism designed and configured for mechanically agitating a trichome-containing plant tissue in a fluid and collecting said fluid containing the trichome exudates and/or content.
Dependencies are inferred from the claim text.