Methanol-responsive promoter, fusion gene comprising promoter and foreign gene connected so that foreign gene can be expressed, vector, transformant, and method of producing protein

Abstract

A methanol-responsive promoter includes the following regions (1) and (2) in this order in the direction from its 5′- to 3′-terminal (1) a methanol-responsive region that is selected from the group consisting of the following (a), (b) and (c) (a) a polynucleotide consisting of the nucleotide sequence of SEQ ID No 1, (b) a polynucleotide consisting of the nucleotide sequence of SEQ ID No 1 wherein one or more nucleotides are deleted, substituted or added and the polynucleotide has methanol-responsive activity, and (c) a polynucleotide which hybridizes, under a stringent condition, to a polynucleotide consisting of nucleotide sequence complementary to all or part of the nucleotide sequence of SEQ ID No 1, and which has methanol-responsive activity, and (2) a core promoter region which is the minimum region necessary for transcription initiation and which has a binding site for RNA polymerase.

Dependencies are inferred from the claim text.