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US 2009/0253159 A1Published application
Ion channel assay methods
AssigneeVertex Pharmaceuticals (San Diego) LLC
InventorsMichael P. Maher, Jesus E. Gonzalez, III
Life of this patent
Estimating the term…
Abstract
A method of characterizing the biological activity of a candidate compound may include exposing cells to the candidate compound, and then exposing the cells to a repetitive application of electric fields so as to set the transmembrane potential to a level corresponding to a pre-selected voltage dependent state of a target ion channel.
Claims21
-20. (canceled)
A method of assaying the biochemical activity of a compound against a target ion channel comprising,
- selecting a cell line having a normal resting transmembrane potential corresponding to a selected voltage dependent state of said target ion channel, wherein said cell line is selected from one or more of CHL, LTK(−), CHO-K1, HEK-293, RBL, F11, and HL5;
- expressing said target ion channel in a population of cells of said selected cell line;
- exposing said population of cells to said compound;
- repetitively exposing said population of cells to one or more electric fields produced with extracellular electrodes so as to effect a controlled change in transmembrane potential of one or more cells of said population of cells;
- and
- monitoring, without using a patch clamp, changes in the transmembrane potential of said one or more cells of said population of cells.
3 more elements
(canceled)
(canceled)
A method of assaying ion channel activity comprising:
- exposing at least one non-excitable cell in solution to a plurality of electric field pulses produced with extracellular electrodes so as to create a controlled change in transmembrane potential and so as to activate an ion channel of interest;
- and
- detecting ion channel activity by detecting one or more changes in transmembrane potential without using a patch clamp.
(canceled)
-48. (canceled)
Dependencies are inferred from the claim text.