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US 2009/0253157 A1Published application
Method of directed differentiation of porcine embryonic stem cells and using the said cells in drug screening
AssigneeLivestock Research Institute Council of Agriculture Executive Yuan
InventorsJenn-Rong Yang, Lih-Ren Chen, Yow-Ling Shiue, Chia-Hsin Liao
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Abstract
The present invention relates to a method of directed differentiation of porcine embryonic stem cells into specific neural lineages. The present invention also relates to a method for identifying neurogenic stimulator using the said porcine embryonic stem cells.
Claims6
A cell system for producing neurons via directed differentiation comprising a porcine embryonic stem (pES) cell line derived from pre-implantation blastocytes.
A method for inducing neural differentiation of embryonic stem (ES) cells in a two-stage protocol comprising:
- (1) culturing ES cells in a suspension culture in ES-cell culture medium (ESM) containing a neurogenic stimulator for a 12-day period;
- (2) collecting the cultured cells from the ESM;
- (3) plating the collected cells onto gelatin-coated dishes and culturing them for an appropriate period to allow differentiation into dopaminergic neurons, cholinergic (ChAT) neurons or GABAergic (GABA) neurons.
Dependencies are inferred from the claim text.