Self-Renewing Master Adult Pluripotent Stem Cells
AssigneeStempeutics Research Private Limited
InventorsSatish Totey, Jyothi Prasanna
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Abstract
The present invention relates to a method for obtaining master adult pluripotent stem (MAPS) cells from adult human corneal epithelial tissues. The MAPS cells are obtained on the basis of pluripotent markers. Further the invention provides MAPS cells that are capable of self renewal and differentiation and have characteristics similar to that of human embryonic stem cells. The MAPS cells also retain the ability to differentiate into cells of different lineages. The composition comprising MAPS cells are useful for therapeutic purposes. Further, the invention provides a culture medium for proliferation of MAPS cells.
Claims16
1-28. (canceled)
Master Adult Pluripotent Stem (MAPS) cells obtained from corneal epithelial tissues, wherein said MAPS cells are capable of self renewal and differentiation.
A culture medium for proliferation of MAPS cells, wherein said culture medium comprises:
- a. basal culture medium;
- and
- b. an effective amount of supplements.
A method of obtaining Master Adult Pluripotent Stem (MAPS) cells from corneal epithelial tissues, said method comprising steps of:
- a. obtaining cells from the corneal epithelial tissues;
- b. culturing the cells of step (a) in a growth culture medium to obtain MAPS cells;
- c. selecting MAPS cells from step (b) based on the expression of pluripotent markers;
- and
- d. culturing MAPS cells of step (c) in a MAPS cell culture medium.
Dependencies are inferred from the claim text.