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US 2009/0253141 A1Published application
Methods and apparatuses for analyzing polynucleotide sequences
AssigneeHelicos BioSciences Corporation
InventorStephen R. Quake
Life of this patent
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Abstract
Methods for high speed, high throughput analysis of polynucleotide sequences, and apparatuses with which to carry out the methods are provided in the invention.
Claims20
A method of conducting single molecule sequencing, the method comprising:
- providing a device comprising a synthesis channel or a reaction chamber,
- introducing in said synthesis channel or reaction chamber reagents necessary of a polymerase-mediated template-dependent nucleic synthesis reaction, comprising a plurality of templates, one or more primers, a polymerase and one or more dye-labeled nucleotides;
- conducting said reaction,
- determining a sequence of the template by detecting individual nucleotides incorporated into a copy of the template immobilized on a surface,
- wherein one or more of the reagents is introduced in the synthesis channel or reaction chamber at a flow rate that does not cause substantial sheering of nucleic acid template and/or dislocation of the polymerase from nucleic acid complexes.
2 more elements
A method of conducting single molecule sequencing, the method comprising:
- providing a device comprising a synthesis channel or a reaction chamber,
- introducing in said synthesis channel or reaction chamber reagents necessary of a polymerase-mediated template-dependent nucleic synthesis reaction, comprising a plurality of templates, one or more primers, a polymerase and one or more dye-labeled nucleotides;
- conducting said reaction,
- determining a sequence of the template by detecting individual nucleotides incorporated into a copy of the template immobilized on a surface,
- wherein one or more of the reagents is introduced in the synthesis channel or reaction chamber at a flow rate that maintains substantially laminar flow in the channel or chamber.
2 more elements
A method of conducting single molecule sequencing, the method comprising:
- providing a device comprising a synthesis channel or a reaction chamber,
- introducing in said synthesis channel or reaction chamber reagents necessary of a polymerase-mediated template-dependent nucleic synthesis reaction, comprising a plurality of templates, one or more primers, a polymerase and one or more dye-labeled nucleotides;
- conducting said reaction,
- determining a sequence of the template by detecting individual nucleotides incorporated into a copy of the template immobilized on a surface,
- wherein one or more of the reagents is introduced into the synthesis channel or reaction chamber at a flow rate below 10 cm/sec.
2 more elements
Dependencies are inferred from the claim text.