Identification of usa300 community-associated methicillin-resistant staphylococcus aureus
AssigneeCreighton University
InventorRichard V. Goering
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Abstract
The present invention provides methods for identifying the US A300 strain of methicillin-resistant Staphylococcus aureus (MRSA).
Claims22
A method for identifying the USA300 strain of methicillin-resistant S. aureus, the method comprising:
- analyzing a methicillin-resistant S. aureus bacterium for the presence or absence of a gene for the Panton-Valentine Leukocidin (PVL) toxin;
- and
- analyzing methicillin-resistant S. aureus DNA for the presence or absence of an AT repeat region in the conserved hypothetical gene SACOL0058;
- wherein the presence of a gene for the PVL toxin and the presence of an AT repeat region comprising at least 6 AT repeats in the conserved hypothetical gene SACOL0058 indicates that the methicillin-resistant S. aureus bacterium is the USA300 strain of methicillin-resistant S. aureus.
An isolated DNA fragment of a methicillin-resistant USA300 S. aureus bacterium gene, wherein the fragment includes an AT repeat region of the conserved hypothetical gene SACOL0058 comprising 6 or more AT repeats.
An isolated oligonucleotide primer having the sequence TGLCTLCGALCGTCAALTALTATATATAT (SEQ ID NO:5) wherein NL represent a locked nucleic acid base.
An isolated oligonucleotide primers selected from the group consisting of 5′-ACGATGATATTCCCGATAG-3′ (SEQ ID NO:3) and 5′-CAATTAACGATGATATTCCCGATAG-3′ (SEQ ID NO:4).
A kit comprising an oligonucleotide primer pair capable of amplifying an AT repeat region of in the conserved hypothetical gene SACOL0058 of S. aureus DNA and an oligonucleotide primer capable of amplifying a gene for the PVL toxin.
Dependencies are inferred from the claim text.