Nucleotide sequence for identifying of acinetobacter bacteria, and method and kit of identification of acinetobacter bacteria
AssigneeCenters for Disease Control Department of Healthy
InventorsShu-Ying Li, Yu-Chi Lin
Life of this patent
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Abstract
Nucleotide primers for identifying Acinetobacter bacteria. The nucleotide primers of the invention are SEQ ID No: 1 to 13, which can be used to effectively and accurately identify various Acinetobacter bacteria. Additionally, the invention further provides a method and kit of identification of Acinetobacter bacteria using the SEQ ID No: 1 to 13.
Claims16
A nucleotide primer for identifying Acinetobacter genomic species, comprising at least one primer selected from a group consisting of SEQ ID NO:
- 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, and a complementary sequence thereof, wherein the SEQ ID NO:1 or a complementary sequence thereof identifies A. calcoaceticus, the SEQ ID NO:2 or a complementary sequence thereof identifies A. baumannii, the SEQ ID NO:3 or a complementary sequence thereof identifies Acinetobacter genomic species 3, the SEQ ID NO:4 or a complementary sequence thereof identifies A. haemolyticus, the SEQ ID NO:5 or a complementary sequence thereof identifies A. junii, the SEQ ID NO:6 or a complementary sequence thereof identifies Acinetobacter genomic species 6, the SEQ ID NO:7 or a complementary sequence thereof identifies A. johnsonii, the SEQ ID NO:8 or a complementary sequence thereof identifies A. lwoffii, the SEQ ID NO:9 or a complementary sequence thereof identifies Acinetobacter genomic species 10, the SEQ ID NO:10 or a complementary sequence thereof identifies Acinetobacter genomic species 11, the SEQ ID NO:11 or a complementary sequence thereof identifies A. radioresistens, the SEQ ID NO:12 or a complementary sequence thereof identifies Acinetobacter genomic species 13TU, and the SEQ ID NO:13 or a complementary sequence thereof identifies Acinetobacter genomic species 16.
A nucleotide primer for identifying A. calcoaceticus-A. baumannii complex, comprising at least one primer selected from a group consisting of SEQ ID NO:
- 1, 2, 3, 12, and a complementary sequence thereof.
A method for identifying Acinetobacter genomic species, comprising:
- obtaining a biological sample from a subject, wherein the biological sample contains a nucleotide of Acinetobacter genomic species;
- amplifying a 16S-23S rDNA intergenic spacer of Acinetobacter genomic species using the nucleotide of Acinetobacter genomic species as a template;
- providing at least one nucleotide primer selected from a group consisting of SEQ ID NO:
- 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, and a complementary sequence thereof;
- performing a primer extension using the 16S-23S rDNA intergenic spacer as a template and the nucleotide primer as a primer;
- and
- determining whether a primer extension product is formed or not, wherein the presence of the primer extension product indicates that the biological sample contains a related Acinetobacter genomic species corresponding to the nucleotide primer.
A kit, comprising:
- one or more primer(s) selected from a group consisting of SEQ ID NO:
- 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, and a complementary sequence thereof, wherein the primer is hybridized to a 16S-23S rDNA intergenic spacer of Acinetobacter genomic species;
- and
- an instruction manual for use of the kit.
Dependencies are inferred from the claim text.