Method for amt-rflp dna fingerprinting

A method for identifying a sample organism, comprising the steps of:

• obtaining a sample of DNA;
• cutting said sample by using a restriction endonuclease that recognizes a specific DNA cut site, wherein cutting produces at least one fragment of said sample;
• treating said cut sample with a phosphatase to remove phosphates from ends of said fragments;
• ligating a double-stranded linker sequence to each end of said fragments;
• adding at least one said fragment to a polymerase chain reaction using a SYBR green-intercalating dye, wherein said fragment is used as a template;
• amplifying said fragments by using one primer complementary to said ligated linker sequence and one primer complementary to a universal DNA sequence, wherein amplification produces at least two strands;
• denaturing at least two said strands at a high temperature;
• lowering said temperature until said strands reassemble, wherein said SYBR green dye is deposited between said strands;
• recording at least three data points as said temperature is slowly increased;
• comparing said data points with known information about organisms;
• and
• determining said identification of said sample organism.