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US 2009/0221007 A1Published application
Diagnosis, prevention and treatment of crohn's disease using the ompc antigen
AssigneesCedars-Sinai Medical Center, The Regents of the University of California
InventorsStephan R. Targan, Jonathan Braun, Christopher L. Sutton
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Abstract
The present invention provides a method of diagnosing Crohn's disease in a subject by determining the presence or absence or IgA anti-OmpC antibodies in the subject, where the presence of the IgA anti-OmpC antibodies indicates that the subject has Crohn's disease.
Claims17
-16. (canceled)
A method for improving the diagnosis of Crohn's disease, said method comprising:
- (a) contacting a sample containing an IgA anti-OmpC antibody with an OmpC antigen or reactive fragment thereof, to transform the IgA anti-OmpC antibody and OmpC antigen into a complex;
- (b) contacting the sample having said complex with an enzyme-labeled anti-IgA antibody to transform the complex into a labeled complex;
- (c) contacting the labeled complex with an enzyme substrate to produce a product;
- and
- (d) detecting said product using a spectrophotometer.
2 more elements
An assay system, said assay system comprising:
- (a) a plate device comprising an OmpC antigen or reactive fragment thereof, wherein said OmpC antigen comprises the amino acid sequence of SEQ ID NO:
- 1 or 90% identity thereof;
- (b) an indicator secondary antibody specific for an OmpC autoantibody;
- and
- (c) instructions for detecting said OmpC autoantibody.
2 more elements
An assay to aid in the detection of Crohn's disease comprising:
- (a) contacting a sample with an OmpC antigen or reactive fragment thereof, under conditions suitable to transform said OmpC antigen into a complex comprising OmpC antigen or reactive fragment thereof, and an IgA anti-OmpC antibody;
- (b) contacting said complex with an enzyme labeled indicator antibody to transform said complex into a labeled complex;
- (c) contacting said labeled complex with a substrate for said enzyme;
- and
- (d) detecting the IgA anti-OmpC antibodies in said sample.
2 more elements
Dependencies are inferred from the claim text.