Set1 proteins and uses thereof
AssigneeAuckland Uniservices Limited
InventorsJohn David Fraser, Ries Langley
Life of this patent
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Abstract
The invention relates to methods of use of SET1 proteins or functional equivalents thereof. More particularly the invention relates to the use of SET1 proteins or functional equivalents thereof in procedure, for identification and/or isolation of IgA and the scrum complement factor C5.
Claims37
A method of isolating IgA present in a sample, the method comprising at least the steps of:
- Bringing SET1 or a functional equivalent thereof in contact with the sample for a period sufficient to allow SET1 or functional equivalent thereof to bind to IgA to form a complex;
- Separating the complex;
- and,
- Releasing IgA from the complex.
A method of isolating C5 present in a sample, the method comprising at least the steps of:
- Bringing SET1 or a functional equivalent thereof in contact with the sample for a period sufficient to allow SET1 or functional equivalent thereof to bind to C5 to form a complex;
- Separating the complex;
- and,
- Releasing C5 from the complex.
A method for isolating IgA from a sample, the method comprising at least the steps of:
- Providing a matrix to which a SET1 protein or functional equivalent is bound;
- Providing a sample;
- Bringing said matrix and said sample into contact for a period sufficient to allow SET1 or functional equivalent thereof to bind to IgA present in the sample;
- and,
- Releasing IgA from the matrix.
A method for isolating C5 from a sample, the method comprising at least the steps of:
- Providing a matrix to which a SET1 protein or functional equivalent is bound;
- Providing a sample;
- Bringing said matrix and said sample into contact for a period sufficient to allow SET1 or functional equivalent thereof to bind to C5 present in the sample;
- and,
- Releasing C5 from the matrix.
A method of detecting IgA in a sample, the method comprising at least the steps of:
- Contacting a sample with SET1 or functional equivalent thereof for a period sufficient to allow SET1 or functional equivalent thereof to bind to IgA;
- and,
- Detecting bound SET1 or functional equivalent thereof.
A method of detecting C5 in a sample, the method comprising at least the steps of:
- Contacting a sample with SET1 or functional equivalent thereof for a period sufficient to allow SET1 or functional equivalent thereof to bind to C5;
- and,
- Detecting bound SET1 or functional equivalent thereof.
A method of removing IgA from a sample, the method comprising at least the steps of:
- Bringing SET1 or a functional equivalent thereof in contact with the sample for a period sufficient to allow SET1 or functional equivalent thereof to bind to IgA to form a complex;
- and,
- Separating the complex from the sample.
A method of removing C5 from a sample, the method comprising at least the steps of:
- Bringing SET1 or a functional equivalent thereof in contact with the sample for a period sufficient to allow SET1 or functional equivalent thereof to bind to C5 to form a complex;
- and,
- Separating the complex from the sample.
An isolated protein having the amino acid sequence SEQ ID NO:
- 6 or SEQ ID NO:
- 7.
An isolated nucleic acid encoding a protein of SEQ ID NO:
- 6 or SEQ ID NO:
- 7.
A kit for the detection, isolation, and/or removal of IgA and/or C5 in a sample, the kit comprising at least SET1 or a functional equivalent thereof.
Dependencies are inferred from the claim text.