Non-signal Imidazole Reagents for Mass Spectrometry Analysis of Phosphomonoesters
AssigneeTrace Bio Analytics, LLC
InventorsPoguang Wang, Guodong Li, Roger W. Giese
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Abstract
Analytical chemical reagents termed non-signal imidazoles and a method for their use that provide a host of advantages for analysis of phosphomonoesters are described. The method and compounds of the invention provide a host of advantages for the analysis of phosphomonoester-containing compounds, namely characteristic, multi-analyte detection with high sensitivity and specificity of known and unknown phosphomonoester-compounds simultaneously.
Claims18
A method for detecting a phosphomonoester-containing compound, said method comprising the steps of:
- providing a sample to be assayed for the presence of a phosphomonoester-containing compound;
- providing a non-signal imidazole reagent, said reagent having an imidazole group, wherein a hydrogen or deuterium atom is attached to one of the two nitrogen atoms of the imidazole group, and wherein at least one of the two adjacent carbon atoms of the imidazole group is substituted with an atom other than hydrogen and wherein the imidazole reagent has no ionic groups;
- combining said sample and said non-signal imidazole reagent, wherein said non-signal imidazole reagent forms a product comprising a phosphorimidazolide anion signal group with any reactable phosphomonoester-containing compound in said sample;
- and
- detecting said product as a phosphorimidazolide anion by mass spectrometry.
A non-signal imidazole reagent, said reagent having the structure:
- wherein either R1 or R2 is hydrogen (H) or deuterium (D), B comprises one to eight carbon atoms, A comprises an aryl group, and at least one of A or B is substituted with one or more atoms or groups other than H when A is phenyl and B comprises an amide group;
- and wherein the imidazole reagent has no ionic groups.
A phosphorimidazolide anion having the structure:
- wherein R1 comprises an aryl group, and R1 has no ionic groups and R2 is an organic molecule.
Dependencies are inferred from the claim text.