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US 2009/0215178 A1Published application
Methods to enhance the stability and homogeneity of transgene expression in clonal cell lines
OwnerZequn Tanginventor
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Abstract
Strategies for increasing the productivity of recombinant protein expression in isogenic cell-lines. Development of a novel selection system for the improved stability and homogeneity of transgene expression in isogenic cells without the dependence on continuous selective drugs.
Claims21
A method to generate cell clones with stable transgene (Gene Of Interest) expression. The method are composed of
- (a.) Inactivation of endogenous essential genes of cell substrate through Gene Inactivation Cassettes (GIC)
- (b.) Trans-complementation of essential genes products through Gene Supplementary Cassettes (GSC), alternatively, the cassette codes for Gene product that capable of inactivating GIC function
- (c.) The GOI expression cassettes are preferably, not necessary, physically linked to GSC to take advantage of favorable selective pressure.
- (d.) Gene Inactivation Cassettes could be preferably, not necessary, physically linked to Gene Supplementary Cassettes and GOI expression cassettes
1 more element
The GIC might target essential genel and GSC supplement with gene 2. The GSC is, preferably but not absolutely necessary, codes for a different (gene 2) as that of inactivated by GIC (gene 1) on the same DNA molecules (plasmid, etc.).
Dependencies are inferred from the claim text.