Methods and Compositions Related to Cyclic Peptide Synthesis
AssigneeUniversity of Utah Research Foundation
InventorsEric W. Schmidt, Brian Hathaway, James T. Nelson
Life of this patent
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Abstract
Disclosed are compositions and methods for cyclization of polymers such as peptides.
Claims47
An isolated peptide comprising an amino acid segment comprising the amino acid sequence of SEQ ID NO:
- 1, an amino acid sequence at least about 90% identical to the amino acid sequence of SEQ ID NO:
- 1, or the amino acid sequence of SEQ ID NO:
- 1 having one or more conservative amino acid substitutions.
An isolated peptide comprising an amino acid segment comprising the amino acid sequence of SEQ ID NO:
- 50, an amino acid sequence at least about 90% identical to the amino acid sequence of SEQ ID NO:50, or the amino acid sequence of SEQ ID NO:50 having one or more conservative amino acid substitutions.
An isolated peptide comprising an amino acid segment comprising the amino acid sequence of SEQ ID NO:
- 2, an amino acid sequence at least about 90% identical to the amino acid sequence of SEQ ID NO:
- 2, or the amino acid sequence of SEQ ID NO:
- 2 having one or more conservative amino acid substitutions.
An isolated peptide comprising an amino acid segment comprising the amino acid sequence of SEQ ID NO:
- 3, an amino acid sequence at least about 90% identical to the amino acid sequence of SEQ ID NO:
- 3, or the amino acid sequence of SEQ ID NO:
- 3 having one or more conservative amino acid substitutions.
An isolated peptide comprising an amino acid segment comprising the amino acid sequence of SEQ ID NO:
- 4, an amino acid sequence at least about 90% identical to the amino acid sequence of SEQ ID NO:
- 4, or the amino acid sequence of SEQ ID NO:
- 4 having one or more conservative amino acid substitutions.
An isolated peptide comprising an amino acid segment comprising the amino acid sequence of SEQ ID NO:
- 5, an amino acid sequence at least about 90% identical to the amino acid sequence of SEQ ID NO:
- 5, or the amino acid sequence of SEQ ID NO:
- 5 having one or more conservative amino acid substitutions.
An isolated peptide comprising an amino acid segment comprising the amino acid sequence of SEQ ID NO:
- 6, an amino acid sequence at least about 90% identical to the amino acid sequence of SEQ ID NO:
- 6, or the amino acid sequence of SEQ ID NO:
- 6 having one or more conservative amino acid substitutions.
An isolated peptide comprising an amino acid segment comprising the amino acid sequence of SEQ ID NO:
- 7, an amino acid sequence at least about 90% identical to the amino acid sequence of SEQ ID NO:
- 7, or the amino acid sequence of SEQ ID NO:
- 7 having one or more conservative amino acid substitutions.
An isolated peptide comprising an amino acid segment comprising the amino acid sequence of SEQ ID NO:
- 8, an amino acid sequence at least about 90% identical to the amino acid sequence of SEQ ID NO:
- 8, or the amino acid sequence of SEQ ID NO:
- 8 having one or more conservative amino acid substitutions.
An isolated peptide comprising an amino acid segment comprising the amino acid sequence of SEQ ID NO:
- 9, an amino acid sequence at least about 90% identical to the amino acid sequence of SEQ ID NO:
- 9, or the amino acid sequence of SEQ ID NO:
- 9 having one or more conservative amino acid substitutions.
A vector comprising a nucleotide sequence encoding a fusion polypeptide comprising, from N-terminus to C-terminus:
- a) a C-terminal domain comprising SEQ ID NO:
- 10;
- b) a peptide;
- c) an N-terminal domain comprising SEQ ID NO:
- 11;
- wherein the fusion polypeptide is able to cyclize the peptide to produce a cyclic peptide in a mammalian cell.
A vector comprising a nucleotide sequence encoding a fusion polypeptide comprising, from N-terminus to C-terminus:
- a) a C-terminal domain comprising SEQ ID NO:
- 11;
- b) a peptide;
- c) an N-terminal domain comprising SEQ ID NO:;
- wherein the fusion polypeptide is able to cyclize the peptide to produce a cyclic peptide in a mammalian cell.
A vector comprising a nucleotide sequence encoding a fusion polypeptide comprising, from N-terminus to C-terminus:
- a) a C-terminal domain comprising SEQ ID NO:
- 10;
- b) a peptide;
- c) an N-terminal domain comprising SEQ ID NO:
- 12;
- wherein the fusion polypeptide is able to cyclize the peptide to produce a cyclic peptide in a mammalian cell.
A vector comprising a nucleotide sequence encoding a fusion polypeptide comprising, from N-terminus to C-terminus:
- a) a C-terminal domain comprising SEQ ID NO:
- 13;
- b) a peptide;
- c) an N-terminal domain comprising SEQ ID NO:
- 14;
- wherein the fusion polypeptide is able to cyclize the peptide to produce a cyclic peptide in a mammalian cell.
A method for cyclizing a polypeptide, the method comprising inserting the polypeptide to be cyclized in the “N1” or “N2” position of SEQ ID NO:
- 1.
A method for cyclizing a polypeptide, the method comprising inserting the polypeptide to be cyclized in the “N” position of SEQ ID NO:
- 2.
A method for cyclizing a polypeptide, the method comprising inserting the polypeptide to be cyclized in the “N” position of SEQ ID NO:
- 3.
A method for cyclizing a polypeptide, the method comprising inserting the polypeptide to be cyclized in the “N” position of SEQ ID NO:
- 9.
A method for cyclizing a polypeptide, the method comprising inserting the polypeptide to be cyclized in the “N1” or “N2” position of SEQ ID NO:
- 50.
A method for prenylating a polypeptide, the method comprising inserting the polypeptide to be cyclized in the “N1” or “N2” position of SEQ ID NO:
- 50.
Dependencies are inferred from the claim text.